The midgut is the digestive apparatus of the silkworm and its proteome was studied by using nano-LC (liquid chromatography) electrospray ionization MS/MS (tandem MS). MS data were analysed by using X!Tandem searching software using different parameters and validated by using the Poisson model. A total of 90 proteins were identified and 79 proteins were described for the first time. Among the new proteins, (i) 22 proteins were closely related to the digestive function of the midgut, including 11 proteins of digestive enzymes secreted by the epithelium, eight proteins of intestine wall muscle and mechanical digestion and three proteins of peritrophic membrane that could prevent the epithelium from being mechanically rubbed; (ii) 44 proteins were involved in metabolism of substance and energy; and (iii) 11 proteins were associated with signal transduction, substance transport and cell skeleton.
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Research Article|
July 22 2009
Identification of the proteome of the midgut of silkworm, Bombyx mori L., by multidimensional liquid chromatography (MDLC) LTQ-Orbitrap MS
Hui-Peng Yao;
Hui-Peng Yao
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Xing-Wei Xiang;
Xing-Wei Xiang
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Lin Chen;
Lin Chen
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Ai-Qin Guo;
Ai-Qin Guo
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Fang-Qing He;
Fang-Qing He
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Li-Pan Lan;
Li-Pan Lan
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Xing-Meng Lu;
Xing-Meng Lu
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Xiao-Feng Wu
Xiao-Feng Wu
1
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
1To whom any correspondence should be addressed (email wuxiaofeng@zju.edu.cn).
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Publisher: Portland Press Ltd
Received:
September 19 2008
Revision Received:
November 10 2008
Accepted:
November 14 2008
Accepted Manuscript online:
November 14 2008
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biosci Rep (2009) 29 (6): 363–373.
Article history
Received:
September 19 2008
Revision Received:
November 10 2008
Accepted:
November 14 2008
Accepted Manuscript online:
November 14 2008
Citation
Hui-Peng Yao, Xing-Wei Xiang, Lin Chen, Ai-Qin Guo, Fang-Qing He, Li-Pan Lan, Xing-Meng Lu, Xiao-Feng Wu; Identification of the proteome of the midgut of silkworm, Bombyx mori L., by multidimensional liquid chromatography (MDLC) LTQ-Orbitrap MS. Biosci Rep 1 December 2009; 29 (6): 363–373. doi: https://doi.org/10.1042/BSR20080144
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