A new lectin gene was isolated by using genomic walker technology and revealed to encode a mannose-binding lectin. Analysis of a 2233 bp segment revealed a gene including a 1169 bp 5′ flanking region, a 417 bp open reading frame (ORF) and a 649 bp 3′ flanking region. There are two putative TATA boxes and eight possible CAAT boxes lie in the 5′ flanking region. The ORF encodes a 15.1 kDa precursor, which contains a 24-amino acid signal peptide. One possible polyadenylation signal is found in the 3′-flanking region. No intron was detected within the region of genomic sequence corresponding to zaa (Zantedeschia aethiopica agglutinin) full-length cDNA, which is typical of other mannose-binding lectin gene that have been reported. The deduced amino acid sequence of the lectin gene coding region shares 49–54% homology with other known lectins. The cloning of this new lectin gene will allow us to further study its structure, expression and regulation mechanisms.
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Research Article|
July 18 2005
Genomic Cloning and Characterization of a Novel Lectin Gene from Zantedeschia aethiopica
Zhonghai Chen;
Zhonghai Chen
1State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, 200433, Shanghai, People's Republic of China. +86-21-65643552 kxtang1@yahoo.comkxtang1@sohu.com
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Xiaofen Sun;
Xiaofen Sun
1State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, 200433, Shanghai, People's Republic of China. +86-21-65643552 kxtang1@yahoo.comkxtang1@sohu.com
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Kexuan Tang
Kexuan Tang
1State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, 200433, Shanghai, People's Republic of China. +86-21-65643552 kxtang1@yahoo.comkxtang1@sohu.com
2Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, 200030, Shanghai, People's Republic of China
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2005 Springer Science+Business Media, Inc.
2005
Biosci Rep (2004) 24 (3): 225–234.
Citation
Zhonghai Chen, Xiaofen Sun, Kexuan Tang; Genomic Cloning and Characterization of a Novel Lectin Gene from Zantedeschia aethiopica. Biosci Rep 1 June 2004; 24 (3): 225–234. doi: https://doi.org/10.1007/s10540-005-2583-4
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