A study was initiated to test whether the FM1–43 dye technique could beapplied to the study of endocytic membrane activity in two rodent prostatecancer (MAT-LyLu and AT-2) cell lines of markedly different metastaticability. The lipophilic dye FM1–43, which has frequently been used tomonitor endo/exocytic activity in excitable cells was employed. We found,as in excitable tissues, that both strongly metastatic (MAT-LyLu) andweakly metastatic (AT-2) cells in culture take up FM1–43 to give vesicularstaining of a variable pattern, which appeared to differ between the twocell lines. However, unlike excitable tissues, neither cell linesubsequently released the dye. Indeed, both cell lines retained the dyethrough several rounds of cell division suggesting that dye incorporatedby cells does not enter the endo/exocytotic cycle. Uptake of dye wasindependent of temperature, Na+/K+ gradients, pH or metabolism. Wesuggest that passive accumulation of FM1–43 can occur in cancer cells andshould not, automatically, be interpreted as evidence of endocytosis.
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Research Article|
February 01 2000
A Study of Membrane Activity in Rat Prostate Cancer Cells: An Evaluation of the FM1–43 Dye Technique
Maria E. Mycielska;
Maria E. Mycielska
1Department of Biology, Neurobiology Group, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, London SW7 2AZ, U.K.
2Department of Cell Biology, Institute of Molecular Biology, Jagiellonian University, al. Mickiewicza 3, 31-120 Krakow, Poland
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Monika Schäfer;
Monika Schäfer
3Institute for Biology II, RWTH Aachen, 52074 Aachen, Germany
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Scott P. Fraser;
Scott P. Fraser
1Department of Biology, Neurobiology Group, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, London SW7 2AZ, U.K.
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C. Lindsay Bashford
C. Lindsay Bashford
4Department of Biochemistry and Immunology, St George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2000 Plenum Publishing Corporation
2000
Biosci Rep (2000) 20 (1): 31–40.
Citation
Maria E. Mycielska, Monika Schäfer, Scott P. Fraser, Mustafa B. A. Djamgoz, C. Lindsay Bashford; A Study of Membrane Activity in Rat Prostate Cancer Cells: An Evaluation of the FM1–43 Dye Technique. Biosci Rep 1 February 2000; 20 (1): 31–40. doi: https://doi.org/10.1023/A:1005531100458
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