Exo- and endocytotic processes induced by depolarization of isolated neurosecretory nerve terminals show a close temporal correlation, which suggests a short time of integration of the neurosecretory granule membrane with the plasma membrane. In order to determine minimal time requirements for exocytosis-coupled endocytosis to occur, we have analyzed by electron microscopy uptake of horserdish peroxidase (HRP) as a fluid phase marker at the onset of depolarization. We have applied rapid mixing and sampling (quenched flow) to assess events in subsecond time peroids after stimulation. A significant number of labelled endocytotic vacuoles was observed during the first second of depolarization. This number then further increased by a factor of about 2 (within 5 s) and 4 (within 50s). Thus, as for exocytosis, the rate of endocytosis decreased considerably during prolonged stimulation. These data indicate i) that a substantial proportion of secretory granules undergoes exocytosis very shortly after stimulation, and ii) that, following exocytosis, the minimal time required for consecutive membrane retrieval is in the sub-second range.
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Research Article|
December 01 1992
Exo-endocytosis in isolated peptidergic nerve terminals occurs in the sub-second range
Gerd Knoll;
Gerd Knoll
2Universität Konstanz Fakultät für Biologie, Postfach 5560, D-7750, Konstanz, Germany
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Helmut Plattner;
Helmut Plattner
2Universität Konstanz Fakultät für Biologie, Postfach 5560, D-7750, Konstanz, Germany
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Jean J. Nordmann
Jean J. Nordmann
1C.N.R.S., Centre de Neurochemie-UPR 416, 5 rue Blaise Pascal, F-67084 Strasborg Cedex, France
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Publisher: Portland Press Ltd
Received:
August 13 1992
Revision Requested:
August 19 1992
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1992 Plenum Publishing Corporation
1992
Biosci Rep (1992) 12 (6): 495–501.
Article history
Received:
August 13 1992
Revision Requested:
August 19 1992
Citation
Gerd Knoll, Helmut Plattner, Jean J. Nordmann; Exo-endocytosis in isolated peptidergic nerve terminals occurs in the sub-second range. Biosci Rep 1 December 1992; 12 (6): 495–501. doi: https://doi.org/10.1007/BF01122037
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