Calpain, a calcium-dependent, neutral cysteine-protease was purified from the erythrocyte cytosol of subjects having essential hypertension (HTN), sickle cell anaemia, (SCA), or kwashiorkor (KWA). Identical electrophoretic mobility on SDS-polyacrylamide gradient gel, sensitivity to micromolar amounts of Ca2+, absolute requirement for a reducing environment and a high susceptibility to inhibition by leupeptin and thiol-group modifying reagents confirm that calpain preparations from these erythrocytes are equivalent to calpain I. Whereas the extent of calpain activation of erythrocyte membrane Ca2+-pumping ATPase of normal subjects was almost equal to that due to calmodulin, calpain activation of the HTN and SCA pump was greater than activation by calmodulin. Like in normal membranes, exogenous calmodulin protected the Ca2+-pumping ATPase of these erythrocytes against calpainization; the degree of protection by calmodulin is least in SCA and HTN. Electrophoretic separation of erythrocyte membranes and the purified Ca2+-pumping ATPase of HTN, SCA and KWA subjects does not indicate the presence of fragments resulting from the proteolytic action of calpain.
Skip Nav Destination
Article navigation
Rapid Communication|
June 01 1990
Comparative action of calpain on erythrocyte Ca2+-pumping ATPase in sickle cell anaemia, essential hypertension and kwashiorkor
O. O. Olorunsogo;
O. O. Olorunsogo
1Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria
Search for other works by this author on:
F. O. Agbolade;
F. O. Agbolade
1Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria
Search for other works by this author on:
S. O. Owojuyigbe;
S. O. Owojuyigbe
1Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria
Search for other works by this author on:
J. A. Adebisi;
J. A. Adebisi
1Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria
Search for other works by this author on:
A. O. Adebayo;
A. O. Adebayo
1Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria
Search for other works by this author on:
W. G. Okunade
W. G. Okunade
1Laboratory for Biomembrane Research, Biochemistry Department, College of Medicine, University of Ibadan, Ibadan, Nigeria
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 24 1990
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1990 Plenum Publishing Corporation
1990
Biosci Rep (1990) 10 (3): 281–291.
Article history
Received:
April 24 1990
Citation
O. O. Olorunsogo, F. O. Agbolade, S. O. Owojuyigbe, J. A. Adebisi, A. O. Adebayo, W. G. Okunade; Comparative action of calpain on erythrocyte Ca2+-pumping ATPase in sickle cell anaemia, essential hypertension and kwashiorkor. Biosci Rep 1 June 1990; 10 (3): 281–291. doi: https://doi.org/10.1007/BF01117244
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.