The Akirin family of transcription cofactors are involved throughout the metazoan in the regulation of different biological processes such as immunity, interdigital regression, muscle and neural development. Akirin do not have catalytic or DNA-binding capability and exert its regulatory function primarily through interacting proteins such as transcription factors, chromatin remodelers, and RNA-associated proteins. In this study, we focused on the human Akirin2 regulome and interactome in neutrophil-like model human Caucasian promyelocytic leukemia HL60 cells. Our hypothesis is that metazoan evolved to have Akirin2 functional complements and different Akirin2-mediated mechanisms for the regulation of gene expression. To address this hypothesis, experiments were conducted using transcriptomics, proteomics and systems biology approaches in akirin2 knockdown and wildtype HL60 cells to characterize Akirin2 gene/protein targets, functional complements and to provide evidence of different mechanisms that may be involved in Akirin2-mediated regulation of gene expression. The results revealed Akirin2 gene/protein targets in multiple biological processes with higher representation of immunity and identified immune response genes as candidate Akirin2 functional complements. In addition to linking chromatin remodelers with transcriptional activation, Akirin2 also interacts with histone H3.1 for regulation of gene expression.
Research Article|
July 05 2021
Function of cofactor Akirin2 in the regulation of gene expression in model human Caucasian neutrophil-like HL60 cells
Sara Artigas-Jerónimo;
Sara Artigas-Jerónimo
Instituto de Investigacion en Recursos Cinegeticos, Ciudad Real, Spain
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Margarita Villar;
Margarita Villar
Instituto de Investigacion en Recursos Cinegeticos, Ciudad Real, Spain
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Agustín Estrada-Peña;
Agustín Estrada-Peña
Faculty of Veterinary Medicine, Spain
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Adrián Velázquez-Campoy;
Adrián Velázquez-Campoy
University of Zaragoza, Zaragoza, Spain
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Pilar Alberdi;
Pilar Alberdi
Instituto de Investigacion en Recursos Cinegeticos, Ciudad Real, Spain
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José de la Fuente
Instituto de Investigacion en Recursos Cinegeticos, Ciudad Real, Spain
* Corresponding Author; email: jose_delafuente@yahoo.com
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Publisher: Portland Press Ltd
Received:
May 08 2021
Revision Received:
June 29 2021
Accepted:
July 02 2021
Online ISSN: 1573-4935
Print ISSN: 0144-8463
Copyright 2021 The Author(s)
2021
This is an Accepted Manuscript; not the final Version of Record. You are encouraged to use the final Version of Record that, when published, will replace this manuscript and be freely available under a Creative Commons licence.
Biosci Rep (2021) BSR20211120.
Article history
Received:
May 08 2021
Revision Received:
June 29 2021
Accepted:
July 02 2021
Citation
Sara Artigas-Jerónimo, Margarita Villar, Agustín Estrada-Peña, Adrián Velázquez-Campoy, Pilar Alberdi, José de la Fuente; Function of cofactor Akirin2 in the regulation of gene expression in model human Caucasian neutrophil-like HL60 cells. Biosci Rep 2021; BSR20211120. doi: https://doi.org/10.1042/BCJ20210294
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