Specific binding of the C-peptide of proinsulin was evaluated using a transplantable NEDH rat islet cell tumour predominantly composed of insulin-secreting B-cells. Cultured tumour B-cells exhibited greater than 90% viability assessed by trypan blue exclusion, and retained the ability to form tumours with accompanying hypoglycaemia and hyperinsulinaemia after reimplantation. During binding experiments with synthetic rat C-peptide I and iodinated tyrosylated rat C-peptide I, turnout B-cells exhibited 54±6% specific binding. Displacement of tracer increased with increasing concentrations of unlabelled rat C-peptide I (0.25–1,000 ng/ml), and the specificity of binding was substantiated by reduced displacement with human C-peptide. Scatchard analysis of specific C-peptide binding revealed a curvilinear plot with upward concavity. The demonstration of specific C-peptide binding to insulin-secreting B-cells provides evidence for a physiological role of proinsulin C-peptide.
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February 01 1986
Specific binding of the C-peptide of proinsulin to cultured B-cells from a transplantable rat islet cell tumor
Peter R. Flatt;
Peter R. Flatt
1Department of Biochemistry, Divisions of Nutrition & Food Science and Clinical Biochemistry, University of Surrey, Guildford GU2 5XH, UK
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Sara K. Swanston-Flatt;
Sara K. Swanston-Flatt
1Department of Biochemistry, Divisions of Nutrition & Food Science and Clinical Biochemistry, University of Surrey, Guildford GU2 5XH, UK
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Shelagh M. Hampton;
Shelagh M. Hampton
1Department of Biochemistry, Divisions of Nutrition & Food Science and Clinical Biochemistry, University of Surrey, Guildford GU2 5XH, UK
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Clifford J. Bailey;
Clifford J. Bailey
2Department of Molecular Sciences, Division of Biology, University of Aston, Birmingham B4 7ET, UK
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Vincent Marks
Vincent Marks
1Department of Biochemistry, Divisions of Nutrition & Food Science and Clinical Biochemistry, University of Surrey, Guildford GU2 5XH, UK
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Publisher: Portland Press Ltd
Received:
December 19 1985
Accepted:
December 19 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (2): 193–199.
Article history
Received:
December 19 1985
Accepted:
December 19 1985
Citation
Peter R. Flatt, Sara K. Swanston-Flatt, Shelagh M. Hampton, Clifford J. Bailey, Vincent Marks; Specific binding of the C-peptide of proinsulin to cultured B-cells from a transplantable rat islet cell tumor. Biosci Rep 1 February 1986; 6 (2): 193–199. doi: https://doi.org/10.1007/BF01115006
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