A rapid and simple method for the isolation of the two chains of ricin is described. The method involves two chromatographic runs, the first on blue-Sepharose and the second on blue dextran-Sepharose. It is moreover shown that the A chain of ricin, but not the B chain, binds to poly(U)-Sepharose.

Keywords:
ricin, A chain, blue-Sepharose, blue dextran-Sepharose, poly(U)-Sepharose, ribosome-inactivating proteins
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© 1986 Plenum Publishing Corporation
1986
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