Many studies have shown that epigenetic regulation of ERs (oestrogen receptors) plays a key role in the pathogenesis of leukaemia. In the present study, it was found that the methylated status of ERα-A might serve as an epigenetic biomarker of leukaemias. In this study, the protein expression and cell apoptosis, cycle, proliferation and viability with and without 5-aza-dC (5-aza-2′-deoxycytidine) were evaluated with Western blotting, 3H-TdR (3H-thymidine) incorporation, propidium iodide staining and Trypan Blue staining respectively. The protein expression of ERα was significantly enhanced in all leukaemic cell lines using treatment with the DNA demethylation reagent 5-aza-dC. However, no obvious change in the protein expression of ERβ takes place with 5-aza-dC. And with 5-aza-dC, cell apoptosis, cell cycle, cell proliferation and viability were all inhibited significantly. We also tracked 40 cases of leukaemias with ERα-A methylation (95%; 38 of 40) to observe the prognosis 1 year after chemotherapy treatment. The patients with ERα-A methylation have no obvious symptomatic relief; however, two patients without ERα-A methylation have obtained effective relief. This result suggested that ERα plays a significant role in leukaemogenesis, and the methylated status of ERα-A not only might serve as an epigenetic biomarker of leukaemias for diagnosis, but also has the potential to serve as a predictor of prognosis in leukaemias.
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Research Article|
March 12 2010
Methylation status of oestrogen receptor α-A: a predictor of prognosis in leukaemias
Jie Yao;
Jie Yao
1
*Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China
†Department of Clinical Laboratory Science, Third Military Medical University, Chongqing 400038, People's Republic of China
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Xiao-Bing Zhang;
Xiao-Bing Zhang
1
*Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China
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Xiao-li Zhang;
Xiao-li Zhang
*Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China
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Wei-Ling Fu
Wei-Ling Fu
2
*Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China
2To whom correspondence should be addressed (email weilingfu@yahoo.com).
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Publisher: Portland Press Ltd
Received:
March 27 2009
Revision Received:
June 11 2009
Accepted:
June 25 2009
Accepted Manuscript online:
June 25 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (4): 217–222.
Article history
Received:
March 27 2009
Revision Received:
June 11 2009
Accepted:
June 25 2009
Accepted Manuscript online:
June 25 2009
Citation
Jie Yao, Xiao-Bing Zhang, Xiao-li Zhang, Wei-Ling Fu; Methylation status of oestrogen receptor α-A: a predictor of prognosis in leukaemias. Biosci Rep 1 August 2010; 30 (4): 217–222. doi: https://doi.org/10.1042/BSR20090044
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