Rad23 is an NER (nucleotide excision repair) protein and it plays an important role in the UPP (ubiquitin–proteasome pathway). In the present study, BmRad23 (a homologous gene of Rad23 from Bombyx mori) was cloned and designated as BmRad23. The ORF (open reading frame) of the BmRad23 cDNA encoded deduced 324 amino acids with a calculated molecular mass of 36.13 kDa and an estimated pI of 4.50. The deduced amino acid sequence of the BmRad23 cDNA revealed several indispensable domains for the function of the Rad23 protein family, such as one UbL (ubiquitin-like) region domain and two UBA (ubiquitin-associated) domains. UV irradiation and treatment with chemical DNA-damaging reagent increased the expression of BmRad23. The BmRad23 gene was expressed in all the examined organs, and elevated expression was observed in testis and ovary. Northern blot and immunoblot analyses showed enhanced expression of BmRad23 after day 3 of the wandering stage in the silk gland. From the present results it is suggested that BmRad23 functions in the UPP during the silkworm metamorphosis as well as participating in the NER when the genetic material is damaged by UV irradiation and other genotoxic stresses.
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Research Article|
September 17 2009
Identification and functional characterization of the Rad23 gene of the silkworm, Bombyx mori
He-ping Xu;
He-ping Xu
*Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
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Yu-song Xu;
Yu-song Xu
1
*Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
1To whom any correspondence should be addressed (email xuyusong@zju.edu.cn).
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Hua-bing Wang;
Hua-bing Wang
*Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
†Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi 321-8505, Japan
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Da He;
Da He
*Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
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Hideki Kawasaki
Hideki Kawasaki
†Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi 321-8505, Japan
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Publisher: Portland Press Ltd
Received:
December 05 2008
Revision Received:
January 19 2009
Accepted:
February 09 2009
Accepted Manuscript online:
February 09 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (1): 19–26.
Article history
Received:
December 05 2008
Revision Received:
January 19 2009
Accepted:
February 09 2009
Accepted Manuscript online:
February 09 2009
Citation
He-ping Xu, Yu-song Xu, Hua-bing Wang, Da He, Hideki Kawasaki; Identification and functional characterization of the Rad23 gene of the silkworm, Bombyx mori. Biosci Rep 1 February 2010; 30 (1): 19–26. doi: https://doi.org/10.1042/BSR20080169
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