Initial ligand selection and the intermolecular spatial arrangement ofglycan-lectin complexes are assumed to be essential to induce formationof stable cell aggregates by a lectin. To distinguish effects of thesetwo processes, the tetrameric mistletoe lectin and its isolated B-chainwere used. A reduced impact of multivalency for Ehrlich ascites tumorcells in contrast to rat thymocytes was revealed. Signaling is thusinitiated in a cell-type-dependent manner. Using selective metabolicinhibitors to reduce signal transfer for aggregate stability, decreasein cellular SH-group level was shown to be a common effect accompanyingsuppression of lectin-dependent aggregate stability. The resultsunderscore an intrinsic variability in the relative importance oflectin-dependent glycan aggregation on the cell surface for triggeringpost-binding lectin effects.
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Research Article|
June 01 2000
Cell-Type Dependence of Stability Modulation of Lectin-initiated Contacts by Impairment of Multivalent Carbohydrate Binding and Intracellular Signaling
A. V. Timoshenko;
A. V. Timoshenko
1Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, 220072 Minsk, Belarus
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I. V. Gorudko;
I. V. Gorudko
2Department of Biophysics, Belarusian State University, Pr. F. Skaryny 4, 220050 Minsk, Belarus
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S. André;
S. André
3Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, 80539 Munich, Germany
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H.-J. Gabius
H.-J. Gabius
3Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, 80539 Munich, Germany
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2000 Plenum Publishing Corporation
2000
Biosci Rep (2000) 20 (3): 199–200.
Citation
A. V. Timoshenko, I. V. Gorudko, S. André, H.-J. Gabius; Cell-Type Dependence of Stability Modulation of Lectin-initiated Contacts by Impairment of Multivalent Carbohydrate Binding and Intracellular Signaling. Biosci Rep 1 June 2000; 20 (3): 199–200. doi: https://doi.org/10.1023/A:1005519603863
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