The relationship between Ca2+ transport and energy transduction of myocardial mitochondria in the presence of reactive oxygen species was investigated. Following treatment with oxygen free radicals [superoxide(O2•) or hydroxyl radical (•)OH], lipid free radicals in myocardial mitochondrial membrane could be detected by using the method of EPR spin trap. Simultaneously there were obvious alterations in the free Ca2+ ([Ca2+]m) in the mitochondrial matrix; the physical state of membrane lipid; the efficiency of oxidative phosphorylation (ADP/O); the value of the respiratory control ratio (RCR); and the membrane potential of the inner membrane of myocardial mitochondria. If the concentrations of reactive oxygen species were reduced by about 30%, the alterations in the physical state of the membrane lipid and energy transduction of myocardial mitochondria were not observed, but the changes in Ca2+ homeostasis remained. We conclude that Ca2+ transport by myocardial mitochondria is more sensitive to agents such as (O2•) or •OH, etc. than are oxidation phosphorylation and the respiratory chain.
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Research Article|
December 01 1997
Sensitivity of Ca2+ Transport of Mitochondria to Reactive Oxygen Species
Zhi-wei Yang;
Zhi-wei Yang
1National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101 China.
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Fu-yu Yang
Fu-yu Yang
1National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101 China.
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1997 Plenum Publishing Corporation
1997
Biosci Rep (1997) 17 (6): 557–567.
Citation
Zhi-wei Yang, Fu-yu Yang; Sensitivity of Ca2+ Transport of Mitochondria to Reactive Oxygen Species. Biosci Rep 1 December 1997; 17 (6): 557–567. doi: https://doi.org/10.1023/A:1027316424985
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