We have compared the properties of two N-acyl derivatives of dilauryl phosphatidylethanolamine on lipid polymorphism, vesicle leakage and Sendai virus fusion. The derivatives contained either an N-lauroyl group (NLPE) or an N-acetyl group (NAcPE). Only the NAcPE markedly affected the bilayer to hexagonal transition temperature of dielaidoyl phosphatidylethanolamine, shifting it to higher values. In contrast the NLPE slightly lowered this phase transition temperature. The two lipids also have opposite effects on leakage from small unilamellar vesicles of egg phosphatidylcholine. The NLPE inhibits leakage, while the NAcPE promotes it. This vesicle stabilizing effect of NLPE against leakage is not manifested in alterations of rates or extents of Sendai virus fusion to liposomes of egg phosphatidylethanolamine plus 2% ganglioside GD1a. The NLPE has no effect, while the NAcPE reduces the observed fusion, at least in part as a consequence of a reduction in the final extent of fusion. These results demonstrate that the bilayer stabilizing effects of NLPE do not result in a lower rate of viral fusion. Furthermore, these bilayer stabilizing effects against leakage are not solely a function of the lipid headgroup but also require a structure with three long acyl chains. The reduced leakage is not related to a loss in monolayer curvature strain.
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Research Article|
August 01 1997
Effect of N-acyl-phosphatidylethanolamine on the Membrane Fusion Between Sendai Virus and Liposome
Yoshikatsu Ogawa;
Yoshikatsu Ogawa
1Biomolecule Department, National Institute of Bioscience and Human-Technology, 1-1 Higashi, Tsukuba 305, Japan.
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Richard M. Epand
Richard M. Epand
2Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5.
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1997 Plenum Publishing Corporation
1997
Biosci Rep (1997) 17 (4): 401–408.
Citation
Yoshikatsu Ogawa, Richard M. Epand; Effect of N-acyl-phosphatidylethanolamine on the Membrane Fusion Between Sendai Virus and Liposome. Biosci Rep 1 August 1997; 17 (4): 401–408. doi: https://doi.org/10.1023/A:1027309401361
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