Protein kinase C (PK-C) and casein kinase II (CK-II) activities were studied in two human colon carcinoma cell lines (HT-29 and CaCO-2) undergoing differentiation in vitro resulting, in small-intestine-like cells. CaCo-2 cells, when grown under standard conditions, appear to undergo spontaneous differentiation. In these cells PK-C and CK-II activities were determined on day 5, 10 and 15. No significant differences in activities were seen either in PK-C or CK-II activity. HT-29 cells, when grown in glucose-free medium can be stimulated to undergo differentiation which is completed within 20 days. PK-C and CK-II activities were determined after 5, 10, 15, 20 and 25 days, respectively. PK-C activity rose from 7.9±3.5 pmole32P/mg protein/min at day 5 to 37.5±14.8 pmole32P/mg protein/min at day 20. After 25 days the activity was reduced to 20.0±7.8 pmole32P/mg protein/min. CK-II activity did not change significantly during day 5 to 20, but on day 25 there was a significant decrease in CK-II activity from 94.9±6.4 pmole32P/mg protein/min (day 20) to 62.6±3.9 pmole32P/mg protein/min (day 25) p=0.003. The results in this study indicate a role for PK-C and CK-II in cell growth and differentiation.
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June 01 1990
Protein kinase C and casein kinase II activities in two human colon carcinoma cell lines, HT-29 and CaCo-2: Possible correlation with differentiation
Ewa Rydell;
Ewa Rydell
1Department of Pharmacology, Faculty of Health Sciences, University of Linköping, S-581 85 Linköping, Sweden
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Karl-Eric Magnusson;
Karl-Eric Magnusson
2Department of Medical Microbiology, Faculty of Health Sciences, University of Linköping, S-581 85 Linköping, Sweden
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Anita Sjö;
Anita Sjö
2Department of Medical Microbiology, Faculty of Health Sciences, University of Linköping, S-581 85 Linköping, Sweden
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Krister Axelsson
Krister Axelsson
1Department of Pharmacology, Faculty of Health Sciences, University of Linköping, S-581 85 Linköping, Sweden
3Department of Biology, University of Linköping, S-581 85 Linköping, Sweden
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Publisher: Portland Press Ltd
Received:
February 26 1990
Revision Requested:
April 02 1990
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1990 Plenum Publishing Corporation
1990
Biosci Rep (1990) 10 (3): 293–299.
Article history
Received:
February 26 1990
Revision Requested:
April 02 1990
Citation
Ewa Rydell, Karl-Eric Magnusson, Anita Sjö, Krister Axelsson; Protein kinase C and casein kinase II activities in two human colon carcinoma cell lines, HT-29 and CaCo-2: Possible correlation with differentiation. Biosci Rep 1 June 1990; 10 (3): 293–299. doi: https://doi.org/10.1007/BF01117245
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