...D.J. Rigden; M. Herman; S. Gillies; P.A.M. Michels Autophagy is the process by which cellular components are directed to and degraded in the vacuole or lysosome and has been studied largely in yeasts. We present here an in silico genomic analysis of trypanosomatid autophagy aimed at highlighting...

... is primarily exerted post-transcriptionally. Here, we use the recently completed genome sequences of three trypanosomatids, Trypanosoma brucei , Trypanosoma cruzi and Leishmania major , in an in silico analysis of their fundamental RNA polymerase complexes. The core complement of Pol II subunits, including...

... components, and are now studying enzyme–ligand complexes of the first enzyme, trypanothione reductase. Also with regard to trypanosomatids, a question that arose was: why do anti-folates not provide useful therapies? The enzyme pteridine reductase has been shown to contribute to anti-folate drug resistance...

... of the effector sites of the Leishmania and yeast enzymes reveals the structural basis for the different effector specificity. Two loops, comprising residues 443–453 and 480–489, adopt very different conformations in the two enzymes, and Lys-453 and His-480 that are a feature of trypanosomatid enzymes provide...