... cells into mature CD4 + or CD8 + T-lymphocytes. We have characterized the requirements for differentiation within these cultures and used this system to compare the ability of CD34 + cells derived from different sources to produce mature thymocytes. The TREC (T-cell receptor excision circle) assay...

... thymocyte The TCR (T-cell receptor) triggers sequential activation of the tyrosine kinases Lck and ZAP-70 [ζ-chain (TCR)-associated protein kinase of 70 kDa], which together nucleate and activate a signalling complex composed of the transmembrane adaptor LAT (linker for activation of T-cells), Vav...

... autoimmunity knockout mouse phosphoinositide 3-kinase (PI3K) regulatory T-cell (Treg) T-cell thymocyte Due to space constraints, I refer the reader to other reviews with a detailed description of PI3K isoform structure, regulation and activation mechanisms in T-cells [ 1 , 2 ]. To briefly...