...) ultimately contain regions of interest, at which tilt series may be acquired (right image). ( C ) The viral structures contained within the subsequent tomograms can then be solved to subnanometer resolutions by subtomogram averaging. Figure adapted from Zhang et al. [ 106...

...–5 nm) sufficient to make out the shapes and arrangements of large macromolecules [ 7 ]. To obtain higher-resolution structural information, repeating features within the tomogram can be computationally extracted (sub-tomograms), aligned, and averaged, a process known as ‘subtomogram averaging’ (STA...

... array cryo-electron tomography cryoEM in-situ structure subtomogram averaging All motile bacteria and archaea examined to date possess a highly conserved chemosensory pathway that monitors the chemical environment and directs cell migration towards nutrient sources, a behavior known...

... that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility. cryo-electron microscopy cryo-electron tomography subtomogram averaging Figure 1. An overview of structural...