... the technical developments that now enable mRNA transcripts and their translation to be visualized at single-molecule resolution in both fixed and live cells. These tools include single-molecule fluorescence in situ hybridization (smFISH) to visualize mRNA in fixed cells, MS2/PP7 labelling for live mRNA imaging...

... under the Creative Commons Attribution License 4.0 (CC BY) . chromosomes condensin DNA binding single-molecule SMC structural biology Organising DNA throughout the cell cycle to ensure correct gene expression, DNA replication, and chromosome division is a remarkable feat. Two...

... techniques based on single-molecule detection that have helped overcome this challenge. These studies focus on the assembly dynamics and splicing kinetics in real-time, which help understanding of spliceosomal assembly and catalysis. 1 To whom correspondence should be addressed (email david.rueda...