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Keywords: single-molecule
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Articles
Biochem Soc Trans (2021) 49 (5): 2221–2227.
Published: 08 September 2021
... the technical developments that now enable mRNA transcripts and their translation to be visualized at single-molecule resolution in both fixed and live cells. These tools include single-molecule fluorescence in situ hybridization (smFISH) to visualize mRNA in fixed cells, MS2/PP7 labelling for live mRNA imaging...
Articles
Biochem Soc Trans (2020) 48 (5): 2089–2100.
Published: 02 October 2020
... under the Creative Commons Attribution License 4.0 (CC BY) . chromosomes condensin DNA binding single-molecule SMC structural biology Organising DNA throughout the cell cycle to ensure correct gene expression, DNA replication, and chromosome division is a remarkable feat. Two...
Articles
Biochem Soc Trans (2014) 42 (4): 1211–1218.
Published: 11 August 2014
... techniques based on single-molecule detection that have helped overcome this challenge. These studies focus on the assembly dynamics and splicing kinetics in real-time, which help understanding of spliceosomal assembly and catalysis. The experiments described in the present review utilizing sm-FRET...