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Keywords: single-channel kinetics
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Biochem Soc Trans (2005) 33 (5): 1003–1007.
Published: 26 October 2005
... for at least some fraction of the protein's duty cycle. To determine at which stage of the gating cycle ATP binding is required, we measured the [ATP] dependence of single-channel kinetic parameters. After pre-phosphorylation, patches were exposed to a range of [ATP], each test interposed between...