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Keywords: single molecule
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Articles
Biochem Soc Trans (2021) 49 (3): 1121–1132.
Published: 18 May 2021
... as a ‘needle in the haystack’ challenge. The TF search process is now well understood in bacteria, but its characterization in eukaryotes needs to account for the complex organization of the nuclear environment. Here we review how live-cell single molecule tracking is starting to shed light on the TF search...
Articles
Biochem Soc Trans (2021) 49 (1): 431–439.
Published: 18 February 2021
... in the near future. Correspondence: M. A. B. Baker ( matthew.baker@unsw.edu.au ) 28 10 2020 15 12 2020 20 1 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 DNA-paint fluorescence microscopy single molecule single...
Articles
Biochem Soc Trans (2020) 48 (3): 1057–1066.
Published: 05 May 2020
...Jacob S. Lewis; Alessandro Costa This review discusses recent advances in single-particle cryo-EM and single-molecule approaches used to visualise eukaryotic DNA replication reactions reconstituted in vitro . We comment on the new challenges facing structural biologists, as they turn to describing...
Articles
Biochem Soc Trans (2018) 46 (3): 729–740.
Published: 05 June 2018
... CRISPR DNA fluorescence microscopy single molecule super resolution Observing DNA inside cells has an increasing number of applications ranging from diagnostics [ 1 ], cancer and cell viability research [ 2 – 7 ] to more fundamental studies on chromatin structure and dynamics [ 8 – 11...
Articles
Biochem Soc Trans (2017) 45 (3): 759–769.
Published: 15 June 2017
...Flynn R. Hill; Enrico Monachino; Antoine M. van Oijen The single-molecule approach seeks to understand molecular mechanisms by observing biomolecular processes at the level of individual molecules. These methods have led to a developing understanding that for many processes, a diversity...
Articles
Biochem Soc Trans (2015) 43 (2): 179–185.
Published: 07 April 2015
... of these proteins in industrial applications. We provide a review of recent developments which have utilized single molecule force spectroscopy to mechanically manipulate proteins from extremophilic organisms and the information which has been gained about their stability, flexibility and underlying energy...
Articles
Biochem Soc Trans (2009) 37 (4): 702–706.
Published: 22 July 2009
...Liming Ying At present, technical hurdles remain in probing biochemical processes in living cells and organisms at nanometre spatial resolution, millisecond time resolution and with high specificity and single-molecule sensitivity. Owing to its unique shape, size and electrical properties...
Articles
Biochem Soc Trans (2006) 34 (5): 983–988.
Published: 25 October 2006
... and conformational information about molecules in their cellular context. By recording the spatial trajectories of pairs of interacting molecules, formation of larger molecular complexes can be studied. In the future, multicolour and multiparameter imaging of single molecules in live cells will be a powerful...
Articles
Biochem Soc Trans (2004) 32 (5): 753–756.
Published: 26 October 2004
...H. Li; L. Ying; X. Ren; S. Balasubramanian; D. Klenerman Single-molecule fluorescence has the capability to detect properties buried in ensemble measurements and, hence, provides new insights about biological processes. Ratiometric methods are normally used to reduce the effects of excitation beam...