...-coding RNAs. Ribosome profiling entails the sequencing of ribosome-bound fragments (RBFs) from mRNAs, in order to map translated sequences within the transcriptome [ 11 , 13 ]. Elongating mRNA-associated ribosomes are trapped by a translation inhibitor, followed by nuclease treatment to generate...

... and exonic sites bound by splicing regulators in mouse brain tissue. Ribosome profiling has been used to quantify ribosome density on budding yeast mRNAs under different environmental conditions. Post-transcriptional regulation in neurons requires high spatial and temporal precision, as is evident from...