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Keywords: reverse transfection
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Biochem Soc Trans (2005) 33 (6): 1407–1408.
Published: 26 October 2005
... of each spot on the glass slide become transfected (reverse transfection) with plasmid DNA. After 2 or 3 days, the cell monolayer is fixed and the expressed proteins are detected using anti-His antibodies. Compartmentalization of the proteins is determined by counterstaining each cellular compartment...