... of GTP binding is not to act as a conformational regulator, but rather to function as a stabilizer intrinsic to maintaining the fold of the domain. Pseudokinases can be classified into subgroups based on ATP binding and phosphorylation activity [ 30 ]. In a similar manner, we propose...

... oxidative stress . J. Biol. Chem. 291 , 23366 – 23373 doi: 10.1074/jbc.M116.747881 5 Jacobsen , A.V. and Murphy , J.M. ( 2017 ) The secret life of kinases: insights into non-catalytic signalling functions from pseudokinases . Biochem. Soc. Trans. In press 6 Eyers , P.A...

...Daniel M. Foulkes; Dominic P. Byrne; Fiona P. Bailey; Patrick A. Eyers Tribbles (TRIB) proteins are pseudokinase mediators of eukaryotic signalling that have evolved important roles in lipoprotein metabolism, immune function and cellular differentiation and proliferation. In addition...

... by the adenine ring of ATP. In the present paper, we show how the spine concept can be applied to B-Raf, specifically to engineer a kinase-dead pseudokinase. To achieve this, we mutated one of the C-spine residues in the N-lobe (N-terminal lobe), Ala 481 , to phenylalanine. This mutant cannot bind ATP...

...Hua Zhang; Chuay Yeng Koo; Justin Stebbing; Georgios Giamas Protein kinases play a pivotal role in regulating many aspects of biological processes, including development, differentiation and cell death. Within the kinome, 48 kinases (~10%) are classified as pseudokinases owing to the fact...

...Jeroen Claus; Angus J.M. Cameron; Peter J. Parker Pseudokinases, the catalytically impaired component of the kinome, have recently been found to share more properties with active kinases than previously thought. In many pseudokinases, ATP binding and even some activity is preserved, highlighting...

...Jason S. Kerr; Catherine H. Wilson Pseudokinases are a class of kinases which are structurally designated as lacking kinase activity. Despite the lack of kinase domain sequence conservation, there is increasing evidence that a number of pseudokinases retain kinase activity and/or have critical...

...Isabelle S. Lucet; Jeffrey J. Babon; James M. Murphy Approximately 10% of the human kinome has been classified as pseudokinases due to the absence of one or more of three motifs known to play key roles in the catalytic activities of protein kinases. Structural and functional studies are now...

... an overall sequence homology with protein kinases, the putative kinase activity of ILK has been controversial. Already the initial inspection of the sequence of the KD had raised suspicions that ILK might be a pseudokinase. The ILK KD contains the lysine residue in subdomain II that is required...

...Jeannine M. Mendrola; Fumin Shi; Jin H. Park; Mark A. Lemmon As with other groups of protein kinases, approximately 10% of the RTKs (receptor tyrosine kinases) in the human proteome contain intracellular pseudokinases that lack one or more conserved catalytically important residues. These include...

...Patrick A. Eyers; James M. Murphy Recent studies of proteins containing kinase-like domains that lack catalytic residue(s) classically required for phosphotransfer, termed pseudokinases, have uncovered important roles in cell signalling across the kingdoms of life. Additionally, mutations within...

...Olli Silvennoinen; Daniela Ungureanu; Yashavanthi Niranjan; Henrik Hammaren; Rajintha Bandaranayake; Stevan R. Hubbard JAK (Janus kinase) 2 plays a critical role in signal transduction through several cytokine receptors. JAKs contain a typical tyrosine kinase domain preceded by a pseudokinase [JH2...