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Keywords: protein engineering
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Articles
Biochem Soc Trans (2022) 50 (5): 1505–1516.
Published: 28 October 2022
... it an adaptable gene editing system, it has many characteristics that could be improved for use in biotechnology. Cas9 exhibits significant off-target activity and low on-target nuclease activity in certain contexts. Scientists have undertaken ambitious protein engineering campaigns to bypass these limitations...
Articles
Biochem Soc Trans (2021) 49 (6): 2821–2830.
Published: 23 November 2021
... ( ywjung@kaist.ac.kr ) 8 10 2021 28 10 2021 2 11 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 protein assembly protein engineering protein nanostructures Proteins are versatile, major building blocks...
Articles
Biochem Soc Trans (2021) 49 (6): 2891–2901.
Published: 16 November 2021
... deglycosylation glycosylation O -GlcNAc post translational modification protein engineering O- GlcNAc is a monosaccharide post-translational modification (PTM) installed on thousands of nucleocytoplasmic and mitochondrial proteins at multiple serine or threonine residues in eukaryotic cells [ 1...
Articles
Biochem Soc Trans (2021) 49 (6): 2749–2765.
Published: 08 November 2021
... and the Biochemical Society under a transformative agreement with CAUL. agricultural bioscience imaging techniques membrane proteins nanotechnology pore-forming toxins protein engineering Pore-forming proteins (PFPs) represent a highly diverse and growing class of molecules identified in all...
Articles
Biochem Soc Trans (2020) 48 (6): 2643–2655.
Published: 26 November 2020
... NanoLuc-based sensors and switches with a focus on the underlying protein engineering approaches. We discuss the advantages and disadvantages of various strategies with respect to sensor sensitivity, modularity, and dynamic range of the sensor and provide a perspective on future strategies...
Articles
Biochem Soc Trans (2020) 48 (5): 2205–2212.
Published: 20 October 2020
...@jhu.edu ) 21 7 2020 28 9 2020 30 9 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 protein engineering protein switches transcription factors We start by considering protein switches that are engineered through...
Articles
Biochem Soc Trans (2020) 48 (3): 1153–1165.
Published: 15 June 2020
... enzymes with naturally evolved peptide ligation activity. Here, we provide a review of key natural peptide ligases and proteases engineered to favor peptide ligation activity. We cover the protein engineering approaches used to generate and improve these tools, along with recent biological applications...
Articles
Biochem Soc Trans (2019) 47 (6): 1773–1780.
Published: 05 December 2019
... protein engineering to in silico design to the use of new chemistry types. Such potential is already being shown with the simple peptide-based system (reviewed by Beesley and Woolfson [ 85 ]). We should also consider incorporating different molecular types, even including abiological materials...
Articles
Biochem Soc Trans (2019) 47 (3): 793–800.
Published: 07 May 2019
...-review, we have focused on efforts to engineer enzymes that disrupt quorum sensing by inactivating acyl-homoserine lactone signalling molecules. We review different approaches for protein engineering and provide examples of how these engineering approaches have been used to tailor the stability...
Articles
Biochem Soc Trans (2019) 47 (2): 701–711.
Published: 22 March 2019
... of the protein, i.e. regions remote from the active- or ligand-binding site, is often ignored. However, there is a growing body of literature that reports on, and rationalises, the successful engineering of proteins at remote sites. This minireview will discuss the current state of the art in protein engineering...
Articles
Biochem Soc Trans (2019) 47 (1): 449–460.
Published: 19 February 2019
... 21 1 2019 28 1 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 phage phage tail-like bacteriocin protein engineering receptor-binding protein synthetic biology tailocin Bacteriophages (phages) are bacteria...
Articles
Biochem Soc Trans (2018) 46 (6): 1541–1549.
Published: 31 October 2018
... way of working with membrane proteins was to optimise the environment to suit the protein, through the use of different detergents, solubilising additives, and other adaptations. However, with innovative protein engineering methodologies, the membrane proteins themselves are now being adapted to suit...
Articles
Biochem Soc Trans (2018) 46 (1): 1–9.
Published: 22 December 2017
... of the Biochemical Society 2018 electron transfer protein engineering STM Approximately a third of all proteins are metalloproteins, integrating one or more metal ions within their molecular structure. The ion(s) plays crucial structural and functional roles, one of the most important being...
Articles
Biochem Soc Trans (2017) 45 (3): 831–837.
Published: 15 June 2017
... of the Biochemical Society 2017 allosteric regulation azobenzene molecular machine photoswitch protein engineering Re-engineering of biological materials to control cellular and molecular functions via external stimuli is of great interest in the field of Synthetic Biology and might have useful...
Articles
Biochem Soc Trans (2016) 44 (4): 1165–1175.
Published: 15 August 2016
... biology [ 4 ] or xenobiology [ 9 , 10 ]. Beyond improving our understanding of life and its origins, these modifications can be applied to novel biocontainment strategies [ 9 – 11 ], therapeutic agents [ 12 , 13 ] and protein engineering [ 14 ]. Partitioning of a diversified population...
Articles
Biochem Soc Trans (2016) 44 (3): 790–795.
Published: 09 June 2016
... of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) . membrane proteins protein engineering protein stability protein structure Integral membrane proteins (IMPs) exist within lipid membranes. Current estimates suggest that between 15 and 30% of open...
Articles
Biochem Soc Trans (2016) 44 (3): 689–691.
Published: 09 June 2016
... Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) . biotechnology molecular modelling protein design protein engineering synthetic biology BrisSynBio is supported by £13.6 million from Biotechnology and Biological Sciences Research Council (BBSRC...
Articles
Biochem Soc Trans (2016) 44 (1): 129–142.
Published: 09 February 2016
... 2015 © 2016 Authors; published by Portland Press Limited 2016 carbohydrate directed evolution enzymatic synthesis glycosyltransferase mutagenesis protein engineering A growing appreciation for the important biological roles and the therapeutic potential of carbohydrates has...
Articles
Biochem Soc Trans (2015) 43 (5): 881–888.
Published: 09 October 2015
...., 30 March 2015–1 April 2015. References 1 Matouschek A. Kellis J.T. Serrano L. Fersht A.R. Mapping the transition state and pathway of protein folding by protein engineering Nature 1989 340 122 126 10.1038/340122a0 2739734 2 Tang K.S. Fersht A.R...
Articles
Biochem Soc Trans (2013) 41 (5): 1195–1200.
Published: 23 September 2013
... is properly cited. bacterial enhancer binding protein cell signalling chimaeric protein protein engineering synthetic biology two-component signalling system Most bacteria live in a continuously changing environment. In order to survive, they have evolved many signalling pathways to sense...
Articles
Biochem Soc Trans (2013) 41 (5): 1177–1182.
Published: 23 September 2013
... be addressed to either of these authors (email reddingtonsc@cf.ac.uk or jonesdd@cf.ac.uk ). 31 5 2013 © 2013 The Authors 2013 Click chemistry expanded genetic code p -azido- L -phenylalanine phenyl azide photocontrol protein engineering Uaas (unnatural amino acids) provide...
Articles
Biochem Soc Trans (2013) 41 (5): 1201–1205.
Published: 23 September 2013
... 2013 The Authors 2013 fluorescent sensor Förster resonance energy transfer (FRET) intracellular imaging protein engineering synthetic biology The availability of robust and generally applicable strategies to construct protein switches is vital for many areas of life sciences...
Articles
Biochem Soc Trans (2013) 41 (5): 1305–1311.
Published: 23 September 2013
... © 2013 The Authors 2013 di-iron carboxylate protein electron transfer protein engineering respiration ubiquinol oxidase It is generally recognized that the distribution of the AOX (alternative oxidase) is substantially wider than previously thought [ 1 ]. In addition to being...
Articles
Biochem Soc Trans (2013) 41 (5): 1189–1194.
Published: 23 September 2013
..., distribution and reproduction in any medium, provided the original work is properly cited. antibody engineering gene library MAX randomization overlap PCR protein engineering saturation mutagenesis Saturation mutagenesis (replacement of wild-type codons with codons for all 20 amino acids...
Includes: Supplementary data
Articles
Biochem Soc Trans (2012) 40 (3): 561–566.
Published: 22 May 2012
... oxidoreductase protein design protein engineering synthetic protein All too commonly, it is inferred that a specific biochemical function is necessarily linked with either a singular enzyme structure or a particular primary sequence. This ignores the apparent ‘memory-less’ random Markovian process...
Articles
Biochem Soc Trans (2009) 37 (4): 717–721.
Published: 22 July 2009
... specificity and action. Rational and combinatorial protein engineering techniques allow introduction of additional features into these synthetic growth molecules, as well as natural growth factors, which significantly enhance their therapeutic utility. 1 To whom correspondence should be addressed...
Articles
Biochem Soc Trans (2008) 36 (6): 1106–1111.
Published: 19 November 2008
... from [ 33 ], © 1998 American Chemical Society. © The Authors Journal compilation © 2008 Biochemical Society 2008 abzyme de novo protein design haem maquette protein engineering water penetration Most work regarding the assembly of novel catalytic function has concentrated...
Articles
Biochem Soc Trans (2007) 35 (6): 1610–1615.
Published: 23 November 2007
... disadvantages in the large-scale production of chirally pure compounds. We have applied two routes of protein engineering studies to improve the properties of these two enzymes, namely DNA shuffling and site-directed mutagenesis. Altering the substrate specificity of bs LDH by DNA shuffling and changing...
Articles
Biochem Soc Trans (2006) 34 (3): 435–437.
Published: 22 May 2006
... Society 2006 anti-inflammatory drug chemokine mutant glycomics glycosaminoglycan G-protein-coupled receptor (GPCR) protein engineering Chemokines induce cell migration and activation by binding to specific cell surface, seven-transmembrane-spanning GPCRs (G-protein-coupled receptors...
Articles
Biochem Soc Trans (2006) 34 (2): 301–303.
Published: 20 March 2006
... in relation to the choice to be made. Recent developments in cell and protein engineering as well as reactor and process engineering are discussed in addition. The use of immobilization enables isolated enzymes to be effectively recovered from a biocatalytic reaction and recycled. For commercial...
Articles
Biochem Soc Trans (2005) 33 (5): 924–930.
Published: 26 October 2005
...-cooling. detergent lipid membrane protein protein engineering reaction centre X-ray crystallography Three of the modelled LDAO molecules depicted in Figure 1 (D) are located in positions equivalent to the molecule of GGD reported previously [ 50 ] ( Figure 1 B). We have examined...
Articles
Biochem Soc Trans (2005) 33 (4): 851–857.
Published: 01 August 2005
... mutation in the two species). 31 3 2005 © 2005 The Biochemical Society 2005 bacteriochlorophyll electron transfer membrane protein photosynthetic bacteria protein engineering reaction centre Despite the structural symmetry seen in purple bacterial reaction centres...
Articles
Biochem Soc Trans (2003) 31 (3): 558–562.
Published: 01 June 2003
... Focused Meeting held at St. Andrews University, 12–14 January 2003 7 March 2003 © 2003 Biochemical Society 2003 chloroaromatic compound crystal structure cytochrome P450 enzyme P450 monooxygenase protein engineering terpene Abbreviations used: PeCB, pentachlorobenzene...
Articles
Biochem Soc Trans (2001) 29 (2): 147–152.
Published: 01 May 2001
... be addressed (e-mail Simon.Daff@ed.ac.uk ) 15 11 2000 © 2001 Biochemical Society 2001 autoinhibitory domain cytochrome P450 nitric oxide protein engineering NOS, nitric oxide synthase nNOS, iNOS and eNOS, neuronal, induced and endothelial NOS P450, cytochrome P450 BM3, Bacillus...
Articles
Biochem Soc Trans (2000) 28 (6): 961–964.
Published: 01 December 2000
... To whom correspondence should be addressed (e-mail roscoe@univ-perp.fr ) 21 6 2000 © 2000 Biochemical Society 2000 protein engineering LPAAT, lysophosphatidic acid acyltransferase Biotechnological Aspects: Complex Lipids 5 Eccleston, V. S. and Harwood, J. L. ( 1995) Biochim...