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Keywords: protein engineering
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Articles
Biochem Soc Trans (2021) 49 (6): 2821–2830.
Published: 23 November 2021
... ( ywjung@kaist.ac.kr ) 8 10 2021 28 10 2021 2 11 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 protein assembly protein engineering protein nanostructures Proteins are versatile, major building blocks...
Articles
Biochem Soc Trans (2021) 49 (6): 2891–2901.
Published: 16 November 2021
... deglycosylation glycosylation O -GlcNAc post translational modification protein engineering O- GlcNAc is a monosaccharide post-translational modification (PTM) installed on thousands of nucleocytoplasmic and mitochondrial proteins at multiple serine or threonine residues in eukaryotic cells [ 1...
Articles
Biochem Soc Trans (2021) 49 (6): 2749–2765.
Published: 08 November 2021
... challenges, problems, and approaches to studying PFPs. agricultural bioscience imaging techniques membrane proteins nanotechnology pore-forming toxins protein engineering In addition to being a platform for PFPs to bind and insert into, lipid membranes of different physical properties can...
Articles
Biochem Soc Trans (2020) 48 (6): 2643–2655.
Published: 26 November 2020
... NanoLuc-based sensors and switches with a focus on the underlying protein engineering approaches. We discuss the advantages and disadvantages of various strategies with respect to sensor sensitivity, modularity, and dynamic range of the sensor and provide a perspective on future strategies...
Articles
Biochem Soc Trans (2020) 48 (5): 2205–2212.
Published: 20 October 2020
... © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 protein engineering protein switches transcription factors Chimeric transcription factors have also recently been used as a tool to aid in natural product discovery [ 31 ]. Genome...
Articles
Biochem Soc Trans (2020) 48 (3): 1153–1165.
Published: 15 June 2020
... enzymes with naturally evolved peptide ligation activity. Here, we provide a review of key natural peptide ligases and proteases engineered to favor peptide ligation activity. We cover the protein engineering approaches used to generate and improve these tools, along with recent biological applications...
Articles
Biochem Soc Trans (2019) 47 (6): 1773–1780.
Published: 05 December 2019
... 2019 15 11 2019 18 11 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 expanded genetic code oligomerization protein complexes protein engineering structural biology synthetic biology The vast expanse of protein...
Articles
Biochem Soc Trans (2019) 47 (3): 793–800.
Published: 07 May 2019
...-review, we have focused on efforts to engineer enzymes that disrupt quorum sensing by inactivating acyl-homoserine lactone signalling molecules. We review different approaches for protein engineering and provide examples of how these engineering approaches have been used to tailor the stability...
Articles
Biochem Soc Trans (2019) 47 (2): 701–711.
Published: 22 March 2019
... of the protein, i.e. regions remote from the active- or ligand-binding site, is often ignored. However, there is a growing body of literature that reports on, and rationalises, the successful engineering of proteins at remote sites. This minireview will discuss the current state of the art in protein engineering...
Articles
Biochem Soc Trans (2019) 47 (1): 449–460.
Published: 19 February 2019
... 21 1 2019 28 1 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 phage phage tail-like bacteriocin protein engineering receptor-binding protein synthetic biology tailocin Bacteriophages (phages) are bacteria...
Articles
Biochem Soc Trans (2018) 46 (6): 1541–1549.
Published: 31 October 2018
... way of working with membrane proteins was to optimise the environment to suit the protein, through the use of different detergents, solubilising additives, and other adaptations. However, with innovative protein engineering methodologies, the membrane proteins themselves are now being adapted to suit...
Articles
Biochem Soc Trans (2018) 46 (1): 1–9.
Published: 22 December 2017
... of the Biochemical Society 2018 electron transfer protein engineering STM Approximately a third of all proteins are metalloproteins, integrating one or more metal ions within their molecular structure. The ion(s) plays crucial structural and functional roles, one of the most important being...
Articles
Biochem Soc Trans (2017) 45 (3): 831–837.
Published: 15 June 2017
... of the Biochemical Society 2017 allosteric regulation azobenzene molecular machine photoswitch protein engineering The fundamental basis of the ‘molecular tweezer’ engineering strategy lies in the photo-physical properties of the azobenzene group. It exists in two isomerization states...
Articles
Biochem Soc Trans (2016) 44 (4): 1165–1175.
Published: 15 August 2016
... evolution protein engineering selection methodologies synthetic biology synthetic nucleic acid (XNA) xenobiology Directed evolution is a well-established approach for optimizing and engineering novel functions in both nucleic acids and proteins. It has been remarkably successful in isolating...
Articles
Biochem Soc Trans (2016) 44 (3): 790–795.
Published: 09 June 2016
... of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) . membrane proteins protein engineering protein stability protein structure Integral membrane proteins (IMPs) exist within lipid membranes. Current estimates suggest that between 15 and 30% of open...
Articles
Biochem Soc Trans (2016) 44 (3): 689–691.
Published: 09 June 2016
... in synthetic biology. biotechnology molecular modelling protein design protein engineering synthetic biology ...
Articles
Biochem Soc Trans (2016) 44 (1): 129–142.
Published: 09 February 2016
... be addressed (email xiichen@ucdavis.edu ). 18 11 2015 © 2016 Authors; published by Portland Press Limited 2016 carbohydrate directed evolution enzymatic synthesis glycosyltransferase mutagenesis protein engineering A growing appreciation for the important biological roles...
Articles
Biochem Soc Trans (2015) 43 (5): 881–888.
Published: 09 October 2015
... that even small perturbations can re-route the folding transitions. Below, we describe the main findings and conclusions reached to date. Using a protein engineering approach known as ‘phi-value analysis’, the transition states for folding of many globular and a number of repeat proteins have been...
Articles
Biochem Soc Trans (2013) 41 (5): 1195–1200.
Published: 23 September 2013
... is properly cited. bacterial enhancer binding protein cell signalling chimaeric protein protein engineering synthetic biology two-component signalling system Most bacteria live in a continuously changing environment. In order to survive, they have evolved many signalling pathways to sense...
Articles
Biochem Soc Trans (2013) 41 (5): 1177–1182.
Published: 23 September 2013
... electron transfer across a single protein–molecule bridge [ 38 ]. The use of phenyl azide chemistry will expand the routes and specificity of the protein–material interfaces, including with new emerging carbon allotrope materials. Protein Engineering: New Approaches and Applications: A joint...
Articles
Biochem Soc Trans (2013) 41 (5): 1201–1205.
Published: 23 September 2013
... 2013 The Authors 2013 fluorescent sensor Förster resonance energy transfer (FRET) intracellular imaging protein engineering synthetic biology The availability of robust and generally applicable strategies to construct protein switches is vital for many areas of life sciences...
Articles
Biochem Soc Trans (2013) 41 (5): 1305–1311.
Published: 23 September 2013
... © 2013 The Authors 2013 di-iron carboxylate protein electron transfer protein engineering respiration ubiquinol oxidase Figure 1 Structure of TAO and comparison of its active site with other di-iron carboxylate proteins ( A ) Cartoon representation of the dimeric structure...
Articles
Biochem Soc Trans (2013) 41 (5): 1189–1194.
Published: 23 September 2013
..., distribution and reproduction in any medium, provided the original work is properly cited. antibody engineering gene library MAX randomization overlap PCR protein engineering saturation mutagenesis For this longer loop, both right-hand and left-hand acceptors were employed to build two...
Includes: Supplementary data
Articles
Biochem Soc Trans (2012) 40 (3): 561–566.
Published: 22 May 2012
.... Figure 2 Common biological redox cofactors and related novel cofactors incorporated into maquettes © The Authors Journal compilation © 2012 Biochemical Society 2012 electron transfer maquette oxidoreductase protein design protein engineering synthetic protein All too commonly...
Articles
Biochem Soc Trans (2009) 37 (4): 717–721.
Published: 22 July 2009
... specificity and action. Rational and combinatorial protein engineering techniques allow introduction of additional features into these synthetic growth molecules, as well as natural growth factors, which significantly enhance their therapeutic utility. As with the receptor-binding module, oligomerization...
Articles
Biochem Soc Trans (2008) 36 (6): 1106–1111.
Published: 19 November 2008
... novo protein design have illustrated how such control can be incorporated into the design process and have paved the way for the synthesis of nascent enzymatic activity in these systems. abzyme de novo protein design haem maquette protein engineering water penetration Figure 3 Haem...
Articles
Biochem Soc Trans (2007) 35 (6): 1610–1615.
Published: 23 November 2007
... disadvantages in the large-scale production of chirally pure compounds. We have applied two routes of protein engineering studies to improve the properties of these two enzymes, namely DNA shuffling and site-directed mutagenesis. Altering the substrate specificity of bs LDH by DNA shuffling and changing...
Articles
Biochem Soc Trans (2006) 34 (3): 435–437.
Published: 22 May 2006
... Society 2006 anti-inflammatory drug chemokine mutant glycomics glycosaminoglycan G-protein-coupled receptor (GPCR) protein engineering Chemokines induce cell migration and activation by binding to specific cell surface, seven-transmembrane-spanning GPCRs (G-protein-coupled receptors...
Articles
Biochem Soc Trans (2006) 34 (2): 301–303.
Published: 20 March 2006
... in relation to the choice to be made. Recent developments in cell and protein engineering as well as reactor and process engineering are discussed in addition. 1 email j.woodley@ucl.ac.uk 25 11 2005 © 2006 The Biochemical Society 2006 biocatalytic process catalyst cost cell...
Articles
Biochem Soc Trans (2005) 33 (5): 924–930.
Published: 26 October 2005
... in determining the near-exclusive use of the A-branch of cofactors for electron transfer [ 50 ]. 1 To whom correspondence should be addressed (email m.r.jones@bristol.ac.uk ). 8 6 2005 © 2005 The Biochemical Society 2005 detergent lipid membrane protein protein engineering...
Articles
Biochem Soc Trans (2005) 33 (4): 851–857.
Published: 01 August 2005
... be addressed (email m.r.jones@bristol.ac.uk ). bacteriochlorophyll electron transfer membrane protein photosynthetic bacteria protein engineering reaction centre Publication of the X-ray crystal structure of the Rhodopseudomonas viridis reaction centre in the mid-1980s revealed...
Articles
Biochem Soc Trans (2003) 31 (3): 558–562.
Published: 01 June 2003
... Focused Meeting held at St. Andrews University, 12–14 January 2003 7 March 2003 © 2003 Biochemical Society 2003 chloroaromatic compound crystal structure cytochrome P450 enzyme P450 monooxygenase protein engineering terpene Abbreviations used: PeCB, pentachlorobenzene...
Articles
Biochem Soc Trans (2001) 29 (2): 147–152.
Published: 01 May 2001
... III) have been exchanged. Despite its very different flavin redox potentials, the BM3 reductase domain was able to support low levels of CaM-dependent NO synthesis, whereas the NOS reductase domain did not effectively substitute for that of cytochrome P450 BM3. Protein Engineering of Peroxidases...
Articles
Biochem Soc Trans (2000) 28 (6): 961–964.
Published: 01 December 2000
... To whom correspondence should be addressed (e-mail roscoe@univ-perp.fr ) 21 6 2000 © 2000 Biochemical Society 2000 protein engineering LPAAT, lysophosphatidic acid acyltransferase Biotechnological Aspects: Complex Lipids 5 Eccleston, V. S. and Harwood, J. L. ( 1995) Biochim...