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Keywords: protein engineering
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Articles
Biochem Soc Trans (2023) 51 (4): 1585–1595.
Published: 11 July 2023
... 22 6 2023 26 6 2023 biosensors chemical biology fluorescence microscopy fluorescent proteins protein engineering signaling The 1994 demonstration that the green fluorescent protein (GFP) could be recombinantly and functionally expressed in practically any cell type [ 1–3...
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Biochem Soc Trans (2022) 50 (5): 1505–1516.
Published: 28 October 2022
... it an adaptable gene editing system, it has many characteristics that could be improved for use in biotechnology. Cas9 exhibits significant off-target activity and low on-target nuclease activity in certain contexts. Scientists have undertaken ambitious protein engineering campaigns to bypass these limitations...
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Biochem Soc Trans (2021) 49 (6): 2821–2830.
Published: 23 November 2021
...-lattices through interactions via zinc ions [ 37 ]. Correspondence: Yongwon Jung ( ywjung@kaist.ac.kr ) 8 10 2021 28 10 2021 2 11 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 protein assembly protein...
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Biochem Soc Trans (2021) 49 (6): 2891–2901.
Published: 16 November 2021
... deglycosylation glycosylation O -GlcNAc post translational modification protein engineering O- GlcNAc is a monosaccharide post-translational modification (PTM) installed on thousands of nucleocytoplasmic and mitochondrial proteins at multiple serine or threonine residues in eukaryotic cells [ 1...
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Biochem Soc Trans (2021) 49 (6): 2749–2765.
Published: 08 November 2021
... and the Biochemical Society under a transformative agreement with CAUL. agricultural bioscience imaging techniques membrane proteins nanotechnology pore-forming toxins protein engineering Pore-forming proteins (PFPs) represent a highly diverse and growing class of molecules identified in all...
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Biochem Soc Trans (2020) 48 (6): 2643–2655.
Published: 26 November 2020
... NanoLuc-based sensors and switches with a focus on the underlying protein engineering approaches. We discuss the advantages and disadvantages of various strategies with respect to sensor sensitivity, modularity, and dynamic range of the sensor and provide a perspective on future strategies...
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Biochem Soc Trans (2020) 48 (5): 2205–2212.
Published: 20 October 2020
...@jhu.edu ) 21 7 2020 28 9 2020 30 9 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 protein engineering protein switches transcription factors Protein switches exist across all domains of life, serving...
Articles
Biochem Soc Trans (2020) 48 (3): 1153–1165.
Published: 15 June 2020
... enzymes with naturally evolved peptide ligation activity. Here, we provide a review of key natural peptide ligases and proteases engineered to favor peptide ligation activity. We cover the protein engineering approaches used to generate and improve these tools, along with recent biological applications...
Articles
Biochem Soc Trans (2019) 47 (6): 1773–1780.
Published: 05 December 2019
... 2019 15 11 2019 18 11 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 expanded genetic code oligomerization protein complexes protein engineering structural biology synthetic biology The vast expanse of protein...
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Biochem Soc Trans (2019) 47 (3): 793–800.
Published: 07 May 2019
...-review, we have focused on efforts to engineer enzymes that disrupt quorum sensing by inactivating acyl-homoserine lactone signalling molecules. We review different approaches for protein engineering and provide examples of how these engineering approaches have been used to tailor the stability...
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Biochem Soc Trans (2019) 47 (2): 701–711.
Published: 22 March 2019
... of the protein, i.e. regions remote from the active- or ligand-binding site, is often ignored. However, there is a growing body of literature that reports on, and rationalises, the successful engineering of proteins at remote sites. This minireview will discuss the current state of the art in protein engineering...
Articles
Biochem Soc Trans (2019) 47 (1): 449–460.
Published: 19 February 2019
... 21 1 2019 28 1 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 phage phage tail-like bacteriocin protein engineering receptor-binding protein synthetic biology tailocin Bacteriophages (phages) are bacteria...
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Biochem Soc Trans (2018) 46 (6): 1541–1549.
Published: 31 October 2018
... way of working with membrane proteins was to optimise the environment to suit the protein, through the use of different detergents, solubilising additives, and other adaptations. However, with innovative protein engineering methodologies, the membrane proteins themselves are now being adapted to suit...
Articles
Biochem Soc Trans (2018) 46 (1): 1–9.
Published: 22 December 2017
... of the Biochemical Society 2018 electron transfer protein engineering STM Approximately a third of all proteins are metalloproteins, integrating one or more metal ions within their molecular structure. The ion(s) plays crucial structural and functional roles, one of the most important being...
Articles
Biochem Soc Trans (2017) 45 (3): 831–837.
Published: 15 June 2017
... of the Biochemical Society 2017 allosteric regulation azobenzene molecular machine photoswitch protein engineering The fundamental basis of the ‘molecular tweezer’ engineering strategy lies in the photo-physical properties of the azobenzene group. It exists in two isomerization states...
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Biochem Soc Trans (2016) 44 (4): 1165–1175.
Published: 15 August 2016
... biology [ 4 ] or xenobiology [ 9 , 10 ]. Beyond improving our understanding of life and its origins, these modifications can be applied to novel biocontainment strategies [ 9 – 11 ], therapeutic agents [ 12 , 13 ] and protein engineering [ 14 ]. 1 To whom correspondence should be addressed (email...
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Biochem Soc Trans (2016) 44 (3): 790–795.
Published: 09 June 2016
... the Creative Commons Attribution Licence 4.0 (CC BY) . membrane proteins protein engineering protein stability protein structure This review has so far focused on IMPs which normally reside within lipid membranes and which can, by elaborate protein engineering methods, be made to exist...
Articles
Biochem Soc Trans (2016) 44 (3): 689–691.
Published: 09 June 2016
... in synthetic biology. biotechnology molecular modelling protein design protein engineering synthetic biology © 2016 The Author(s) 2016 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons...
Articles
Biochem Soc Trans (2016) 44 (1): 129–142.
Published: 09 February 2016
... development. 1 To whom correspondence should be addressed (email xiichen@ucdavis.edu ). 18 11 2015 © 2016 Authors; published by Portland Press Limited 2016 carbohydrate directed evolution enzymatic synthesis glycosyltransferase mutagenesis protein engineering A growing...
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Biochem Soc Trans (2015) 43 (5): 881–888.
Published: 09 October 2015
.... 1 Correspondence may be addressed to either author (email e.main@qmul.ac.uk and lsi10@cam.ac.uk ) . 13 5 2015 © 2015 Authors; published by Portland Press Limited 2015 ankyrin protein design protein engineering protein folding tandem-repeat protein tetratricopeptide TPR...
Articles
Biochem Soc Trans (2013) 41 (5): 1195–1200.
Published: 23 September 2013
... on. bacterial enhancer binding protein cell signalling chimaeric protein protein engineering synthetic biology two-component signalling system © 2013 The author(s) 2013 This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) ( http...
Articles
Biochem Soc Trans (2013) 41 (5): 1177–1182.
Published: 23 September 2013
... be addressed to either of these authors (email reddingtonsc@cf.ac.uk or jonesdd@cf.ac.uk ). 31 5 2013 © 2013 The Authors 2013 Click chemistry expanded genetic code p -azido- L -phenylalanine phenyl azide photocontrol protein engineering Uaas (unnatural amino acids) provide...
Articles
Biochem Soc Trans (2013) 41 (5): 1201–1205.
Published: 23 September 2013
... 2013 The Authors 2013 fluorescent sensor Förster resonance energy transfer (FRET) intracellular imaging protein engineering synthetic biology Figure 3 A modular design approach for engineering protein switches Using mutually exclusive interactions between input and output domains...
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Biochem Soc Trans (2013) 41 (5): 1305–1311.
Published: 23 September 2013
... © 2013 The Authors 2013 di-iron carboxylate protein electron transfer protein engineering respiration ubiquinol oxidase It is generally recognized that the distribution of the AOX (alternative oxidase) is substantially wider than previously thought [ 1 ]. In addition to being...
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Biochem Soc Trans (2013) 41 (5): 1189–1194.
Published: 23 September 2013
..., distribution and reproduction in any medium, provided the original work is properly cited. antibody engineering gene library MAX randomization overlap PCR protein engineering saturation mutagenesis For this longer loop, both right-hand and left-hand acceptors were employed to build two...
Includes: Supplementary data
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Biochem Soc Trans (2008) 36 (6): 1106–1111.
Published: 19 November 2008
... The Authors Journal compilation © 2008 Biochemical Society 2008 abzyme de novo protein design haem maquette protein engineering water penetration There is evidence of water exclusion even in this first haem maquette, H10H24. The reduction potential of the haem is pH-dependent and coupled...
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Biochem Soc Trans (2006) 34 (2): 301–303.
Published: 20 March 2006
... in relation to the choice to be made. Recent developments in cell and protein engineering as well as reactor and process engineering are discussed in addition. 1 email j.woodley@ucl.ac.uk 25 11 2005 © 2006 The Biochemical Society 2006 biocatalytic process catalyst cost cell...
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Biochem Soc Trans (2005) 33 (5): 924–930.
Published: 26 October 2005
... had been weakened [ 41 ]. 1 To whom correspondence should be addressed (email m.r.jones@bristol.ac.uk ). 8 6 2005 © 2005 The Biochemical Society 2005 detergent lipid membrane protein protein engineering reaction centre X-ray crystallography The (bacterio...
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Biochem Soc Trans (2005) 33 (4): 851–857.
Published: 01 August 2005
... photosynthetic bacteria protein engineering reaction centre 1 To whom correspondence should be addressed (email m.r.jones@bristol.ac.uk ). Nomenclature: The X-ray crystal structure of the Rhodobacter capsulatus reaction centre has not been determined, but it is thought to be highly similar...
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Biochem Soc Trans (2003) 31 (3): 558–562.
Published: 01 June 2003
... Focused Meeting held at St. Andrews University, 12–14 January 2003 7 March 2003 © 2003 Biochemical Society 2003 chloroaromatic compound crystal structure cytochrome P450 enzyme P450 monooxygenase protein engineering terpene Abbreviations used: PeCB, pentachlorobenzene...
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Biochem Soc Trans (2001) 29 (2): 147–152.
Published: 01 May 2001
... be addressed (e-mail Simon.Daff@ed.ac.uk ) 15 11 2000 © 2001 Biochemical Society 2001 autoinhibitory domain cytochrome P450 nitric oxide protein engineering NOS, nitric oxide synthase nNOS, iNOS and eNOS, neuronal, induced and endothelial NOS P450, cytochrome P450 BM3, Bacillus...
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Biochem Soc Trans (2000) 28 (6): 961–964.
Published: 01 December 2000
... To whom correspondence should be addressed (e-mail roscoe@univ-perp.fr ) 21 6 2000 © 2000 Biochemical Society 2000 protein engineering LPAAT, lysophosphatidic acid acyltransferase Biotechnological Aspects: Complex Lipids 5 Eccleston, V. S. and Harwood, J. L. ( 1995) Biochim...