... interactions isotope labelling magic angle spinning molecular docking protein dynamics Enzyme catalysis is required for the vast majority of biochemical reactions to attain rates compatible with the time scales of living organisms. Highly evolved three-dimensional protein structures confer...

... of the Biochemical Society 2019 molecular conformations protein dynamics protein-tyrosine kinases regulatory mechanisms At the end of the 1800s, photographers had been producing still images for nearly a century. The advent of moving pictures at this time caused a dramatic shift in the viewer's...

... allosteric regulation fractals protein conformation protein dynamics Allostery is a process that regulates the activity of enzymes via binding a modulator at a distal site, sterically different from the chemically active site. It is now well recognized that allostery is a fundamental property...

... of the Biochemical Society 2016 ABC proteins EPR spectroscopy membrane transport protein dynamics spin labelling Membrane transporter proteins comprise a wide variety of proteins which underpin a diverse range of functions–from the import of vital solutes to the efflux of a multitude of drugs...

..., which would lead to bimodal or multimodal distances detected by EPR. 1 Correspondence may be addressed to either author (email Fraser.MacMillan@uea.ac.uk and thomas.stockner@meduniwien.ac.at ) 1 6 2015 EPR MD simulations ABCB1 MsbA DEER PELDOR modelling protein dynamics...

...-translational modifications. A powerful tool to study the regulation of membrane signalling enzymes and complexes is hydrogen deuterium exchange–MS (HDX–MS), a technique that allows for the interrogation of protein dynamics upon membrane binding and recruitment. This review will highlight the theory...

... of York, U.K., 17–19 June 2013. Organized and Edited by Nicholas Brindle (University of Leicester, U.K.), Sandip Patel (University College London, U.K.) and Stephen Yarwood (University of Glasgow, U.K.) allostery cAMP cGMP covariance NMR protein dynamics singular value decomposition (SVD...

...Kristina Lorenzen; Tony Pawson In recent years, HDX-MS (hydrogen–deuterium exchange coupled to MS) on biomolecules has evolved from a niche technique to a powerful method in the investigation of protein dynamics. Protein kinases, in particular, represent an area of active study using this technique...

... compilation © 2012 Biochemical Society 2012 intrinsically disordered protein integrative method nuclear magnetic resonance (NMR) protein dynamics small-angle X-ray scattering (SAXS) IDPs (intrinsically disordered proteins) are nowadays recognized as fundamental elements to explain...

... applications, these investigations provide fundamental insights into protein dynamics. Upon photon absorption, the free p -HBI chromophore undergoes strong twisting around the methylene bridge linking its two cyclic moieties, potentially leading to cis – trans isomerization and accounting for the lack...

...David R. Glowacki; Jeremy N. Harvey; Adrian J. Mulholland One of the most controversial questions in enzymology today is whether protein dynamics are significant in enzyme catalysis. A particular issue in these debates is the unusual temperature-dependence of some kinetic isotope effects for enzyme...

... are located at opposite sites of the β-sandwich. The ‘BC’ (L2) and ‘FG’ loops (L5) of C2 bind Ca 2+ , while the AB, CD and EF loops bind Ca 2+ in Calx-β domains. calcium binding Calx-β magnesium binding protein dynamics protein stability sodium/calcium exchanger (NCX) The NCX (Na + /Ca 2...

... Society 2012 allostery cAMP cGMP chemical shift covariance analysis (CHESCA) exchange protein directly activated by cAMP (Epac) nuclear magnetic resonance (NMR) protein dynamics cAMP is an ancient second messenger that controls a multitude of essential cellular processes by binding...

... and substrate in a reactive configuration from which hydride is transferred by quantum mechanical tunnelling. In the present paper, we review results from experiments that probe the influence of protein dynamics on the chemical step of the reaction catalysed by TmDHFR (DHFR from Thermotoga maritima...

... conformational change dihydrofolate reductase (DHFR) protein dynamics protochlorophyllide oxidoreductase The light-driven enzyme POR (protochlorophyllide oxidoreductase; EC 1.3.1.33) catalyses the reduction of the C-17–C-18 double bond of the D-ring of Pchlide (protochlorophyllide) to produce Chlide...

... that we have spearheaded from our work on quantum mechanical tunnelling effects linked to protein dynamics for H-transfer reactions, we review our recent progress in mapping macroscopic kinetic descriptors to an atomistic understanding of dynamics linked to biological H-tunnelling reactions. 22 6...

... of the Cy3 and Cy5 emission until one component is irreversibly photobleached ( Figure 2 and [ 16 ]). If this FRET pair is used to explore protein dynamics on the same time scale as blinking, then careful control experiments are required to establish the behaviour of the protein rather than...

... decreased the protein mobility, such as freezing, dehydration, application of cross-linking agents, embedment in trehalose amorphous matrices etc. could suppress the ET (electron transfer) between Q A and Q B (see [ 3 , 15 , 16 ] and references cited therein). This review is focused on protein dynamics...