...@imperial.ac.uk ). 29 4 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 co-localization single-molecule spectroscopy (CoSMoS) dynamics fluorescence resonance energy transfer (FRET) pre-mRNA splicing single-molecule fluorescence splicessomal RNA Splicing...

...Elizabeth A. Dunn; Stephen D. Rader U6 snRNA (small nuclear RNA), one of five RNA molecules that are required for the essential process of pre-mRNA splicing, is notable for its high level of sequence conservation and the important role it is thought to play in the splicing reaction. Nevertheless...

...Rebecca Hogg; Joanne C. McGrail; Raymond T. O'Keefe The NineTeen Complex (NTC) of proteins associates with the spliceosome during pre-mRNA splicing and is essential for both steps of intron removal. The NTC and other NTC-associated proteins are recruited to the spliceosome where they participate...

...Lily Novak Frazer; Verity Nancollis; Raymond T. O'Keefe Pre-mRNA splicing is an essential step in gene expression where intron regions are removed and coding exon sequences are joined to form an mRNA for translation. Splicing is catalysed by an RNA–protein complex called the spliceosome. A number...

...J.R. Sanford; J. Ellis; J.F. Cáceres SR proteins (serine- and arginine-rich proteins) are an evolutionarily conserved family consisting of essential pre-mRNA splicing factors. Since their discovery and initial characterization, roles of SR proteins in pre-mRNA splicing and in subsequent steps...

... expression ribonucleoprotein (RNP) herpes simplex virus ICP27 nuclear export pre-mRNA splicing Human HSV-1 (herpes simplex virus type 1) is a nuclear replicating DNA virus. After infection, the 152 kb genome, encoding at least 80 genes, circularizes and is transcribed by the host RNA polymerase...

.... Finally, to discover plant-specific proteins in- volved in splicing and in, for example, rRNA or small nuclear RNA processing, green fluorescent protein-cDNA fusion libraries in viral vectors are being screened. Introduction Precursor mRNA (pre-mRNA) splicing requires the early recognition of pre-mRNA...

... and U5 small nuclear ribo-nucleoprotein particles (snRNPs), and some non-snRNP proteins assemble around three short conserved sequences within the intron in an ordered manner to form the active spliceosome. We aim to provide insight into the molecular details of the mechanism of pre-mRNA splicing through...