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Keywords: photobleaching
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Biochem Soc Trans (2006) 34 (5): 979–982.
Published: 25 October 2006
... emission. But the excitation light for Cy3 excites the Cy5 dark isomer and so it can quickly return to the starting fluorescent acceptor state. This behaviour gives anti-correlated blinking of the Cy3 and Cy5 emission until one component is irreversibly photobleached ( Figure 2 and [ 16 ]). If this FRET...