... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 DNA polymerases DNA replication and recommbination mutagenesis PCNA proten-protein interaction translesion DNA synthesis DNA replication is one of the most basic cellular processes that ensures...

... DNA replication and recombination DNA synthesis and repair mutagenesis phenotypic heterogeneity single-cell analysis The maintenance of genome integrity is crucial for essential cell functions and accurate transfer of the genetic information across generations. For this reason, all...

... 2017 © 2017 The Author(s) 2017 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . cowpea mosaic virus cryo-electron microscopy mutagenesis transient...

... side of the active site to the canopy. The mechanism of hydrogen activation has been dissected by site-directed mutagenesis to identify the catalytic base responsible for splitting molecular hydrogen and possible proton transfer pathways to/from the active site. Previous reported attempts to mutate...

... development. 1 To whom correspondence should be addressed (email xiichen@ucdavis.edu ). 18 11 2015 © 2016 Authors; published by Portland Press Limited 2016 carbohydrate directed evolution enzymatic synthesis glycosyltransferase mutagenesis protein engineering A growing...

... The Authors Journal compilation © 2013 Biochemical Society 2013 alanine scan family A GPCRs family B GPCRs mutagenesis secretin-like GPCR structure–activity relationship Family B GPCRs have a number of conserved proline residues, including a proline towards the middle of TM helix 6...

... lantibiotic mutagenesis nisin Figure 1 Biosynthesis of nisin ( A ) Formation of rings A and B only (see B ) is shown for clarily. Serine and threonine residues of the unmodified core peptide (with N-terminal leader peptide attached; not shown) are dehydrated by NisB, forming dehydroalanine (DHA...

... calcitonin mutagenesis receptor activity-modifying protein secretin-like G-protein-coupled receptor structure–activity relationship GPCRs form a number of families. The best characterized of these are the rhodopsin-like or family A GPCRs. The secretin-like or family B GPCRs form receptors...

...). Previous studies have used a chimaeric and site-specific mutagenesis approach to identify three residues in the putative tenth transmembrane helix of SV2A that, when mutated, alter binding of LEV and related racetam derivatives to SV2A. In the present paper, we report a combined modelling and mutagenesis...

..., and specific interactions between GABA and some of these residues have been determined. In the present paper, I show how a cation–π interaction for one of the binding site residues has allowed the production of models of GABA docked into the binding site, and these orientations are supported by mutagenesis...

... both gene-driven (reverse genetics) and phenotype-driven (forward genetics) approaches. A high-efficiency ENU approach results in approx. 25 functional mutations per genome; most of these will result in hypomorphic alleles. Our group has recently begun using ENU mutagenesis as a tool for understanding...

... perturbations. Random mutagenesis was induced, and individual mutagenized chromosomes were recovered by classic crossing schemes involving phenotypical markers and balancer chromosomes. In wild-type larvae, ferritin is predominantly localized in the brain, in regions of the intestine, in wreath cells...

... mutagenesis translesion synthesis ubiquitin The stability of our genetic material is endangered both by DNA-damaging agents from the environment and by endogenous problems during DNA replication arising from spontaneous damage [ 1 ]. DNA repair pathways, the cell's first line of defence against many...

... for secretin binding based on receptor mutagenesis and photoaffinity labelling studies. Full agonist analogues of secretin incorporating a photolabile moiety at various positions throughout the pharmacophore covalently label residues within this region, while only N-terminal probes have labelled the core...

... by an inhibition, mutagenesis and homology-modelling approach. Crystal structure and homology modelling indicate that both enzymes possess a narrow constriction that separates an entrance lipophilic channel from the active-site cavity. The role of the constriction as a mobile gate that permits substrate passage...

... amphotericin B antifungal activity mutagenesis nystatin sterol yeast After more than 40 years of use in antifungal therapy, polyene macrolides nystatin and amphotericin B ( Figure 1 ) still represent the first line treatment of severe systemic fungal infections and several topical mycoses...

... cAMP regulatory element (CRE)-luciferase glucagon G-protein-coupled receptor (GPCR) mutagenesis receptor activation ternary complex The extended ternary complex model for GPCR (G-protein-coupled receptor) activation suggests that they interchange between inactive (R) and active (R*) states...

... Meeting of the Biochemical Society held at the University of Essex, Colchester, 2–4 July 2003 3 July 2003 © 2003 Biochemical Society 2003 glycation glyoxalase methylglyoxal mutagenesis multidrug resistance tumour Abbreviations used: AGE, advanced glycation end...

... in 2D and 3D, a Biochemical Society Focused Meeting held at University of Nottingham, 19–20 June 2003 20 June 2003 © 2003 Biochemical Society 2003 fluorescence spectroscopy mutagenesis secondary-structure prediction steroid receptor transactivation Abbreviations used...

.... 1 To whom correspondence should be addressed (e-mail jl@mrc-lmb.cam.ac.uk ) 12 4 2001 © 2001 Biochemical Society 2001 conformational change metastable model membranes mutagenesis receptor binding APN, aminopeptidase N Bt, Bacillus thuringiensis Membrane Active...