... the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . Open access for this article was enabled by the participation of University of Minnesota in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society. dynamics microscopy p53 signaling single cell...

...Lucien E. Weiss; Tal Naor; Yoav Shechtman The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations...

.... Understanding how PRRs signal is of critical importance due to their potential as therapeutic targets to ameliorate symptoms of inflammatory diseases. The recent advances in microscopy, such as the discovery of fluorescent proteins and the breaking of the diffraction limit of light, offer a unique opportunity...

... electrophoresis, these strands are pulled out into the agarose, forming a tail which, when stained with a fluorescent dye, can be analysed by fluorescence microscopy. The intensity of this tail reflects the amount of DNA damage sustained. Despite being such an established and widely used assay, there are still...

... bacteria as ‘bags of DNA and proteins’, relatively little has been learnt about how they organize their life processes within cells. The mistaken perception of bacteria cells as unstructured systems arose partly because of the difficulty of performing studies by light microscopy due to their small size...

...D.S. Lidke; P. Nagy; B.G. Barisas; R. Heintzmann; J.N. Post; K.A. Lidke; A.H.A. Clayton; D.J. Arndt-Jovin; T.M. Jovin We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy...