... at the DNA damage sites are caused by the retention of the same molecules or by the continual exchange of different molecules remains unknown. Here, we discuss recent results from quantitative live-cell imaging studies focusing on PARP1 and PARP2's distinct DNA substrate specificities and modes...

... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . artificial intelligence classification live-cell imaging machine learning segmentation super-resolution microscopy A hallmark of human intelligence...

.... Decades of research have revealed a wide range of mechanisms through which repair factors recognize damage and co-ordinate repair processes. In recent years, single-molecule live-cell imaging methods have further enriched our understanding of how repair factors operate in the crowded intracellular...

... pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence...

... correspondence should be addressed (email kpfleger@waimr.uwa.edu.au ). 23 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 β-arrestin bioluminescence resonance energy transfer (BRET) clathrin G-protein-coupled receptor (GPCR) live-cell imaging protein–protein...

...G.I. Mashanov; T.A. Nenasheva; M. Peckham; J.E. Molloy Over the last decade, there have been remarkable developments in live-cell imaging. We can now readily observe individual protein molecules within living cells and this should contribute to a systems level understanding of biological pathways...