...@pitt.edu . 2 11 2015 © 2016 Authors; published by Portland Press Limited 2016 lipid raft microdomain nanoscopy phosphoinositide The plasma membrane (PM) of any eukaryotic cell is a frenetic hub of activity. It mediates a ceaseless exchange of minerals, nutrients and waste...

..., with the greatest inhibition of PMCA4. Besides, cholesterol blocked the inhibitory effect of Aβ, which is consistent with the lack of any Aβ effect on PMCA4 found in cholesterol-enriched lipid rafts isolated from pig brain. These observations suggest that PMCAs are a functional component of the machinery that leads...

... of these structures is discussed. Technological improvements for live cell imaging of membrane components are also reviewed. © The Authors Journal compilation © 2009 Biochemical Society 2009 eukaryotic cell fluorescence microscopy lipid raft membrane lipid order multimolecular assembly supramolecular...

... for organizing plasma membrane domains, such as lipid rafts, or for targeted delivery of glycoproteins to the apical or basolateral surface. Galectin–glycan lattice formation is also involved in regulating the signalling threshold of some cell-surface glycoproteins, including T-cell receptors and growth factor...

... cells expressing PrP c to Cu 2+ results in the rapid endocytosis of the protein. First, PrP c translocates laterally out of detergent-resistant lipid rafts into detergent-soluble regions of the plasma membrane, then it is internalized through clathrin-coated pits. The extreme N-terminal region of PrP c...

... obtained by image analysis (Imaris software). ( B ) PDGF activates a pool of SFK in lipid rafts/caveolae for c- myc induction and DNA synthesis and another pool outside lipid rafts/caveolae for actin assembly. Activation of this second pool is through cross-talk signalling: PDGF recruits S1K (sphingosine...

... involving ACs in lipid rafts, held there by protein–protein interactions with other raft residents, along with CCE channel components, NHE1, gravin, PKA and PDE4D, and in which cAMP kinetics differ dramatically from those that reach the cytosol, and which therefore may evoke distinct signalling consequences...

... ) under different hyphal inducing conditions. Colony morphologies were analysed on solid spider plates and induction of filamentation in liquid media in response to N -acetyl- D -glucosamine and serum. Candida albicans drug resistance ergosterol lipid raft morphogenesis sphingolipid...

... signalling components are organized together in membrane microdomains, in particular lipid rafts, enriched in cholesterol and sphingolipids, and caveolae, a subset of lipid rafts that also possess the protein caveolin, whose scaffolding domain may serve as an anchor for signalling components. Caveolae were...

... for the α-cleavage of PrP C . The amyloidogenic cleavage of APP by the β- and γ-secretases appears to occur preferentially in cholesterol-rich lipid rafts, while the conversion of PrP C into the infectious form PrP Sc also appears to occur in these membrane domains. 1 email n.m.hooper@leeds.ac.uk...

... of serine/threonine (but not tyrosine) phosphatases. Our results imply that destabilization of lipid rafts seemingly affects the association of Hsp90 with the respective serine/threonine phosphatases, thereby increasing the accessibility to PKB/Akt to deactivating phosphatases. We have found recently...

...-treated cells. 1 To whom correspondence should be addressed (email nge@gbf.de ). 8 7 2004 © 2004 The Biochemical Society 2004 cholesterol glycosylphosphatidylinositol anchor lipid raft Listeria monocytogenes listeriolysin O oligomerization Fifteen years after...

... Society Focused Meeting held at New Edinburgh Royal Infirmary, Little France, Nr. Edinburgh, 19–20 November 2003 25 November 2003 © 2004 Biochemical Society 2004 apoptosis Bcl-2 caspase 8 death receptor lipid raft inflammation neutrophil Abbreviations used: DISC...

...C.J. Fielding; P.E. Fielding The plasma membrane of mammalian cells consists of microdomains differing in lipid and protein composition. Two distinct classes of cholesterol/sphingolipid microdomain (caveolae and lipid rafts) are assembly points for transmembrane signalling complexes. Recent...