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Keywords: gene editing
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Biochem Soc Trans (2022) 50 (5): 1505–1516.
Published: 28 October 2022
... it an adaptable gene editing system, it has many characteristics that could be improved for use in biotechnology. Cas9 exhibits significant off-target activity and low on-target nuclease activity in certain contexts. Scientists have undertaken ambitious protein engineering campaigns to bypass these limitations...