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Articles

Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome

Chandani Warnasooriya, David Rueda

Biochem Soc Trans (2014) 42 (4): 1211–1218.

https://doi.org/10.1042/BST20140105

Published: 11 August 2014

... spectroscopy (CoSMoS) dynamics fluorescence resonance energy transfer (FRET) pre-mRNA splicing single-molecule fluorescence splicessomal RNA RNA UK 2014: An Independent Meeting held at Low Wood Hotel, Windermere, U.K., 24–26 January 2014. Organized and Edited by Niki Gray, Gracjan Michlewski...

View article titled, Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome

Articles

cAMP measurements with FRET-based sensors in excitable cells

Katy L. Everett, Dermot M.F. Cooper

Biochem Soc Trans (2012) 40 (1): 179–183.

https://doi.org/10.1042/BST20110616

Published: 19 January 2012

... pH can accompany changes in membrane excitability. cAMP fluorescence resonance energy transfer (FRET) neuron pH Depolarization of excitable cells leads to Ca 2+ influx and significant decreases in intracellular pH [ 10 ]. These changes in pH can be attributed to the actions of the PMCA...

View article titled, cAMP measurements with FRET-based sensors in excitable cells

Articles

Single-molecule FRET reveals nucleotide-driven conformational changes in molecular machines and their link to RNA unwinding and DNA supercoiling

Dagmar Klostermeier

Biochem Soc Trans (2011) 39 (2): 611–616.

https://doi.org/10.1042/BST0390611

Published: 22 March 2011

... ). 3 9 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 ATP hydrolysis C-terminal domain DEAD-box fluorescence resonance energy transfer (FRET) RNA-binding domain RNA-recognition motif Many endergonic reactions in the cell are catalysed using the energy...

View article titled, Single-molecule FRET reveals nucleotide-driven conformational changes in molecular machines and their link to RNA unwinding and DNA supercoiling

Articles

FRET (fluorescence resonance energy transfer) sheds light on transcription

Dina Grohmann, Daniel Klose, Daniel Fielden, Finn Werner

Biochem Soc Trans (2011) 39 (1): 122–127.

https://doi.org/10.1042/BST0390122

Published: 19 January 2011

...-time. 1 To whom correspondence should be addressed (email d.grohmann@ucl.ac.uk ). 9 11 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 fluorescence resonance energy transfer (FRET) RNA polymerase TATA-box-binding protein (TBP) transcription...

View article titled, FRET (fluorescence resonance energy transfer) sheds light on transcription

Articles

Red light, green light: probing single molecules using alternating-laser excitation

Yusdi Santoso, Ling Chin Hwang, Ludovic Le Reste, Achillefs N. Kapanidis

Biochem Soc Trans (2008) 36 (4): 738–744.

https://doi.org/10.1042/BST0360738

Published: 22 July 2008

... a.kapanidis1@physics.ox.ac.uk ). 7 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 alternating-laser excitation (ALEX) biomolecular interaction fluorescence resonance energy transfer (FRET) protein–DNA interaction single-molecule fluorescence total internal...

View article titled, Red light, green light: probing single molecules using alternating-laser excitation

Articles

Molecular probes: insights into design and analysis from computational and physical chemistry

Felicity L. Mitchell, Gabriel E. Marks, Elena V. Bichenkova, Kenneth T. Douglas, Richard A. Bryce

Biochem Soc Trans (2008) 36 (1): 46–50.

https://doi.org/10.1042/BST0360046

Published: 22 January 2008

... computational and experimental approach can yield. 1 To whom correspondence should be addressed (email richard.bryce@manchester.ac.uk ). 18 9 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 computational chemistry fluorescence resonance energy transfer (FRET...

View article titled, Molecular probes: insights into design and analysis from computational and physical chemistry

Articles

Phagosomal acidification: measurement, manipulation and functional consequences

B.E. Steinberg, K.K. Huynh, S. Grinstein

Biochem Soc Trans (2007) 35 (5): 1083–1087.

https://doi.org/10.1042/BST0351083

Published: 25 October 2007

... the transition between early and late phagosomes. 1 To whom correspondence should be addressed, at The Hospital for Sick Children (email sga@sickkids.ca ). 11 6 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 acidification fluorescence resonance energy transfer...

View article titled, Phagosomal acidification: measurement, manipulation and functional consequences

Articles

Assessing GPCR activation using protein complementation: a novel technique for HTS

R.M. Eglen

Biochem Soc Trans (2007) 35 (4): 746–748.

https://doi.org/10.1042/BST0350746

Published: 20 July 2007

... that are markedly free from artefactual interferences. 1 email richard.eglen@perkinelmer.com 2 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 bioluminescence resonance energy transfer (BRET) enzyme fragment complementation (EFC) fluorescence resonance energy...

View article titled, Assessing GPCR activation using protein complementation: a novel technique for HTS

Articles

Neurotensin receptor type 1: Escherichia coli expression, purification, characterization and biophysical study

P.J. Harding, H. Attrill, S. Ross, J.R. Koeppe, A.N. Kapanidis, A. Watts

Biochem Soc Trans (2007) 35 (4): 760–763.

https://doi.org/10.1042/BST0350760

Published: 20 July 2007

...–TrxA–His 10 NTS1B MBP–TeV–rT43NTS1–TeV–TrxA–His 10 NTS1C MBP–TeV–rT43NTS1–CFP–TeV–TrxA–His 10 NTS1Y MBP–TeV–rT43NTS1–YFP–TeV–TrxA–His 10 11 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 fluorescence resonance energy transfer (FRET...

View article titled, Neurotensin receptor type 1: Escherichia coli expression, purification, characterization and biophysical study

Articles

Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes

H. Yoshizaki, K. Aoki, T. Nakamura, M. Matsuda

Biochem Soc Trans (2006) 34 (5): 851–854.

https://doi.org/10.1042/BST0340851

Published: 25 October 2006

... network. Akt fluorescence resonance energy transfer (FRET) green fluorescent protein (GFP) imaging phosphoinositide 3-kinase (PI3K) Ral Small GTPases function as critical molecular switches in a variety of signal transduction cascades. Among them, Rho family GTPases function primarily...

View article titled, Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes

Articles

Restricted diffusion of a freely diffusible second messenger: mechanisms underlying compartmentalized cAMP signalling

M. Zaccolo, G. Di Benedetto, V. Lissandron, L. Mancuso, A. Terrin, I. Zamparo

Biochem Soc Trans (2006) 34 (4): 495–497.

https://doi.org/10.1042/BST0340495

Published: 21 July 2006

... should be addressed (email manuela.zaccolo@unipd.it ). 24 3 2006 © 2006 The Biochemical Society 2006 cAMP signalling cell imaging fluorescence resonance energy transfer (FRET) phosphodiesterase (PDE) restricted diffusion second messenger cAMP is a ubiquitous second messenger...

View article titled, Restricted diffusion of a freely diffusible second messenger: mechanisms underlying compartmentalized cAMP signalling

Articles

Use of single-cell imaging techniques to assess the regulation of cAMP dynamics

D. Willoughby, D.M.F. Cooper

Biochem Soc Trans (2006) 34 (4): 468–471.

https://doi.org/10.1042/BST0340468

Published: 21 July 2006

... into the complexities of this finely tuned messenger system and its dynamic interaction with other signalling pathways. adenylate cyclase (AC) calcium cAMP cyclic nucleotide-gated channel (CNGC) exchange protein directly activated by cAMP (Epac) fluorescence resonance energy transfer (FRET) phosphodiesterase...

View article titled, Use of single-cell imaging techniques to assess the regulation of cAMP dynamics

Articles

Imaging the cAMP-dependent signal transduction pathway

M. Zaccolo, T. Cesetti, G. Di Benedetto, M. Mongillo, V. Lissandron, A. Terrin, I. Zamparo

Biochem Soc Trans (2005) 33 (6): 1323–1326.

https://doi.org/10.1042/BST0331323

Published: 26 October 2005

... The Biochemical Society 2005 biosensor cAMP cell imaging fluorescence resonance energy transfer (FRET) protein kinase A signal transduction cAMP is a ubiquitous second messenger that mediates the action of various hormones and neurotransmitters and influences many cell functions by cross-talk...

View article titled, Imaging the cAMP-dependent signal transduction pathway

Articles

Spatiotemporal regulation of the Pak1 kinase

M.C. Parrini, M. Matsuda, J. de Gunzburg

Biochem Soc Trans (2005) 33 (4): 646–648.

https://doi.org/10.1042/BST0330646

Published: 01 August 2005

... London, U.K.). actin cytoskeleton Cdc42 fluorescence resonance energy transfer (FRET) motility p21-activated kinase (Pak) Rac1 Pak1 (p21-activated kinase 1) is the first identified and most studied member of the Pak family comprising six serine/threonine protein kinases in humans. Pak...

View article titled, Spatiotemporal regulation of the Pak1 kinase

Articles

Mechanism and role of localized activation of Rho-family GTPases in growth factor-stimulated fibroblasts and neuronal cells

K. Kurokawa, T. Nakamura, K. Aoki, M. Matsuda

Biochem Soc Trans (2005) 33 (4): 631–634.

https://doi.org/10.1042/BST0330631

Published: 01 August 2005

... to induce neurites during neuronal development, as has been seen in the induction of membrane ruffles. Cdc42 fluorescence resonance energy transfer (FRET) GTPase-activating protein (GAP) imaging Rac Rho Rho-family GTPases function as critical molecular switches in transducing extracellular...

View article titled, Mechanism and role of localized activation of Rho-family GTPases in growth factor-stimulated fibroblasts and neuronal cells

Articles

Subunit movement in individual H + -ATP synthases during ATP synthesis and hydrolysis revealed by fluorescence resonance energy transfer

M. Börsch, P. Gräber

Biochem Soc Trans (2005) 33 (4): 878–882.

https://doi.org/10.1042/BST0330878

Published: 01 August 2005

... fluorescence resonance energy transfer (FRET) hydrolysis single-molecule fluorescence subunit rotation The H + -ATP synthase from Escherichia coli is a large membrane-bound enzyme that consists of eight different subunits. These subunits comprise two parts: the hydrophilic F 1 part with subunit...

View article titled, Subunit movement in individual H + -ATP synthases during ATP synthesis and hydrolysis revealed by fluorescence resonance energy transfer

Articles

Folding and catalysis of the hairpin ribozyme

T.J. Wilson, M. Nahas, T. Ha, D.M.J. Lilley

Biochem Soc Trans (2005) 33 (3): 461–465.

https://doi.org/10.1042/BST0330461

Published: 01 June 2005

..., in which the 5′-oxygen is the nucleophile that attacks the cyclic phosphate. acid–base catalysis fluorescence resonance energy transfer (FRET) hairpin ribozyme RNA catalysis single-molecule spectroscopy © 2005 The Biochemical Society 2005 Figure 6 Summary of rates of reactions...

View article titled, Folding and catalysis of the hairpin ribozyme

Articles

Development and use of fluorescent nanosensors for metabolite imaging in living cells

M. Fehr, S. Okumoto, K. Deuschle, I. Lager, L.L. Looger, J. Persson, L. Kozhukh, S. Lalonde, W.B. Frommer

Biochem Soc Trans (2005) 33 (1): 287–290.

https://doi.org/10.1042/BST0330287

Published: 01 February 2005

... provided us with the possibility of studying metabolic processes in living organisms. However, at present these technologies do not permit measuring at the subcellular level. The cameleon , a fluorescence resonance energy transfer (FRET)-based nanosensor uses the ability of the calcium-bound form...

View article titled, Development and use of fluorescent nanosensors for metabolite imaging in living cells

Articles

Kinetics of G-protein-coupled receptor signalling and desensitization

C. Krasel, J.-P. Vilardaga, M. Bünemann, M.J. Lohse

Biochem Soc Trans (2004) 32 (6): 1029–1031.

https://doi.org/10.1042/BST0321029

Published: 26 October 2004

... it is possible that GRK phosphorylation of GPCRs depends on an additional conformational change in the receptor, which is not required for either G-protein activation or arrestin binding. © 2004 The Biochemical Society 2004 arrestin fluorescence resonance energy transfer (FRET) G-protein-coupled...

View article titled, Kinetics of G-protein-coupled receptor signalling and desensitization

Articles

Functional ramifications of FRET-detected nascent chain folding far inside the membrane-bound ribosome

A.E. Johnson

Biochem Soc Trans (2004) 32 (5): 668–672.

https://doi.org/10.1042/BST0320668

Published: 26 October 2004

...@tamu.edu ). 23 6 2004 © 2004 The Biochemical Society 2004 endoplasmic reticulum membrane fluorescence spectroscopy fluorescence resonance energy transfer (FRET) nascent membrane protein ribosome translocon Not long after ribosomes had been identified and shown...

View article titled, Functional ramifications of FRET-detected nascent chain folding far inside the membrane-bound ribosome

Articles

Distribution of lipid raft markers in live cells

O.O. Glebov, B.J. Nichols

Biochem Soc Trans (2004) 32 (5): 673–675.

https://doi.org/10.1042/BST0320673

Published: 26 October 2004

... markers. However, measurements of FRET and ratio imaging showed that there was no detectable clustering and no overall enrichment of raft markers in these regions. 8 7 2004 © 2004 The Biochemical Society 2004 clustering fluorescence resonance energy transfer (FRET...

View article titled, Distribution of lipid raft markers in live cells

Articles

Imaging protein–protein interactions in cell motility using fluorescence resonance energy transfer (FRET)

M. Parsons, B. Vojnovic, S. Ameer-Beg

Biochem Soc Trans (2004) 32 (3): 431–433.

https://doi.org/10.1042/bst0320431

Published: 01 June 2004

... fluorescent protein; PAK1, p21-activated kinase-1; PKC, protein kinase C; RFP, red fluorescent protein. actin cytoskeleton cell migration fluorescence lifetime imaging microscopy (FLIM) fluorescence resonance energy transfer (FRET) signalling © 2004 Biochemical Society 2004 3...

View article titled, Imaging protein–protein interactions in cell motility using fluorescence resonance energy transfer (FRET)

Articles

Single-molecule studies of DNA and RNA four-way junctions

S.A. McKinney, E. Tan, T.J. Wilson, M.K. Nahas, A.-C. Déclais, R.M. Clegg, D.M.J. Lilley, T. Ha

Biochem Soc Trans (2004) 32 (1): 41–45.

https://doi.org/10.1042/bst0320041

Published: 01 February 2004

... Chemistry and Biology: The 5th Cambridge Symposium, a Biochemical Society-sponsored meeting held at Queen's College, Cambridge, 31 August–3 September 2003 3 September 2003 © 2004 Biochemical Society 2004 branch migration fluorescence resonance energy transfer (FRET) hairpin ribozyme...

View article titled, Single-molecule studies of DNA and RNA four-way junctions

Articles

A new mechanism for membrane iron transport in Pseudomonas aeruginosa

I.J. Schalk, M. A. Abdallah, F. Pattus

Biochem Soc Trans (2002) 30 (4): 702–705.

https://doi.org/10.1042/bst0300702

Published: 01 August 2002

...-uptake mechanism involves all the proteins Key words: fluorescence resonance energy transfer (FRET), iron uptake, outer membrane protein, siderophore. Abbreviations used: Kdapp, apparent dissociation constant; PaA. pyoverd in. 'To whom comsDondence should be addressed (e-mail generally reported...

View article titled, A new mechanism for membrane iron transport in Pseudomonas aeruginosa

NARROW

Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome

cAMP measurements with FRET-based sensors in excitable cells

Single-molecule FRET reveals nucleotide-driven conformational changes in molecular machines and their link to RNA unwinding and DNA supercoiling

FRET (fluorescence resonance energy transfer) sheds light on transcription

Red light, green light: probing single molecules using alternating-laser excitation

Molecular probes: insights into design and analysis from computational and physical chemistry

Phagosomal acidification: measurement, manipulation and functional consequences

Assessing GPCR activation using protein complementation: a novel technique for HTS

Neurotensin receptor type 1: Escherichia coli expression, purification, characterization and biophysical study

Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes

Restricted diffusion of a freely diffusible second messenger: mechanisms underlying compartmentalized cAMP signalling

Use of single-cell imaging techniques to assess the regulation of cAMP dynamics

Imaging the cAMP-dependent signal transduction pathway

Spatiotemporal regulation of the Pak1 kinase

Mechanism and role of localized activation of Rho-family GTPases in growth factor-stimulated fibroblasts and neuronal cells

Subunit movement in individual H + -ATP synthases during ATP synthesis and hydrolysis revealed by fluorescence resonance energy transfer

Folding and catalysis of the hairpin ribozyme

Development and use of fluorescent nanosensors for metabolite imaging in living cells

Kinetics of G-protein-coupled receptor signalling and desensitization

Functional ramifications of FRET-detected nascent chain folding far inside the membrane-bound ribosome

Distribution of lipid raft markers in live cells

Imaging protein–protein interactions in cell motility using fluorescence resonance energy transfer (FRET)

Single-molecule studies of DNA and RNA four-way junctions

A new mechanism for membrane iron transport in Pseudomonas aeruginosa

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