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Keywords: fluorescence resonance energy transfer
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Biochem Soc Trans (2021) 49 (4): 1685–1694.
Published: 04 August 2021
... resonance energy transfer hydrophobic mismatch PIP2 SMALP Membrane and soluble proteins live in different environments. Soluble proteins are surrounded in all directions by an aqueous solvent. This medium is homogeneous, and is believed to have similar properties throughout the cell [ 1...
Biochem Soc Trans (2020) 48 (1): 103–111.
Published: 03 February 2020
... evolved energy sensing of eukaryotic cells provided by the AMP-activated protein kinase (AMPK). Our synthetic nano-sensor relies on fluorescence resonance energy transfer (FRET) to detect changes in ATP:ADP and ATP:AMP ratios both in vitro and in cells in vivo . Construction and iterative optimisation...
Biochem Soc Trans (2019) 47 (5): 1327–1339.
Published: 11 October 2019
.... Correspondence: Viacheslav O. Nikolaev ( v.nikolaev@uke.de ) 22 7 2019 20 9 2019 24 9 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 biosensor cardiac cGMP fluorescence resonance energy transfer microdomain 3′,5...
Biochem Soc Trans (2004) 32 (5): 753–756.
Published: 26 October 2004
... inhomogeneity. Fluorescence resonance energy transfer is widely used but there are problems in inserting the fluorophores in the correct position on the biomolecule, particularly if the structure is not known. We have recently developed two-colour coincidence single-molecule fluorescence that addresses...
Biochem Soc Trans (2001) 29 (6): 732–736.
Published: 01 November 2001
... on functional testing (5-HT uptake assays, inhibition of 5-HT uptake by blockers such as imipramine). Oligomers were visualized by fluorescence resonance energy transfer (FRET) microscopy in living cells using complementary methods. Interestingly, oligomerization was not confined to hSERT; FRET was also...