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Keywords: fluorescence
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Biochem Soc Trans (2023) 51 (6): 2041–2059.
Published: 28 November 2023
...Hisham Mazal; Franz-Ferdinand Wieser; Vahid Sandoghdar Fluorescence microscopy has witnessed many clever innovations in the last two decades, leading to new methods such as structured illumination and super-resolution microscopies. The attainable resolution in biological samples is, however...
Biochem Soc Trans (2021) 49 (6): 2903–2915.
Published: 08 November 2021
... of apoptosis restoration strategies. Recent years have been prolific in the development of biosensors to visualize cyt c mitochondrial efflux in living cells, by fluorescence microscopy. These biosensors specifically detect endogenous, untagged cyt c , while showing efficient cellular uptake and reduced cell...
Biochem Soc Trans (2018) 46 (3): 729–740.
Published: 05 June 2018
...Lucien E. Weiss; Tal Naor; Yoav Shechtman The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations...
Biochem Soc Trans (2014) 42 (4): 899–904.
Published: 11 August 2014
... 2014 fluorescence nanosensor surface-enhanced Raman spectroscopy (SERS) ratiometric nanosensor 1 To whom correspondence should be addressed (email colin.campbell@ed.ac.uk ). 30 5 2014 Intracellular biological nanosensors are an important tool in the biochemist's...
Biochem Soc Trans (2014) 42 (4): 1211–1218.
Published: 11 August 2014
... components during assembly and catalysis makes it challenging to study the conformations of these components in traditional bulk studies, which makes single-molecule methods particularly powerful [ 13 , 14 ]. Single-molecule fluorescence microscopy allows detection of interactions between molecules...
Biochem Soc Trans (2012) 40 (4): 635–639.
Published: 20 July 2012
...Dejian Zhou Over the last 10 years, fluorescent semiconductor QD (quantum dot)–biomolecule conjugates have emerged as a powerful new sensing platform showing great potential in a wide range of applications in biosensing, environmental monitoring and disease diagnosis. The present mini-review...
Includes: Supplementary data
Biochem Soc Trans (2011) 39 (5): 1221–1225.
Published: 21 September 2011
...Balaraman Kalyanaraman HE (hydroethidine), a widely used fluorescent dye for detecting intracellular superoxide, undergoes specific oxidation and hydroxylation reactions. The reaction between HE and O 2 •− (superoxide radical) yields a diagnostic marker product, 2-hydroxyethidium. This is contrary...
Biochem Soc Trans (2011) 39 (1): 393–398.
Published: 19 January 2011
... is an HPLC-based method with glycans, free or enzymatically released, fluorescently labelled with 2-AA (2-anthranilic acid) [ 20 , 21 ]. The reproducibility and ease of analysis allows this technique to be applied in any experimental or diagnostic laboratory. FOS are formed in the cell as a result...
Biochem Soc Trans (2007) 35 (6): 1579–1582.
Published: 23 November 2007
... should be related to the decrease in the catalytic efficiency. The instability of the fold for the mutant proteins resulted in the accumulation of an intermediate state, partly unfolded, in-between native and unfolded states. Quenching of tryptophan fluorescence by acrylamide has further revealed...
Biochem Soc Trans (2007) 35 (5): 970–973.
Published: 25 October 2007
... (yellow fluorescent protein). YFP PCA was successfully applied to visualize the protein interactions of the cargo transport receptor ERGIC-53 (endoplasmic reticulum–Golgi intermediate compartment protein of 53 kDa) with its luminal interaction partner MCFD2 (multiple coagulation factor deficiency protein...
Biochem Soc Trans (2005) 33 (5): 905–909.
Published: 26 October 2005
...A.M. Powl; J. Carney; P. Marius; J.M. East; A.G. Lee Interactions between a membrane protein and the lipid molecules that surround it in the membrane are important in determining the structure and function of the protein. These interactions can be pictured at the molecular level using fluorescence...
Biochem Soc Trans (2004) 32 (5): 753–756.
Published: 26 October 2004
...H. Li; L. Ying; X. Ren; S. Balasubramanian; D. Klenerman Single-molecule fluorescence has the capability to detect properties buried in ensemble measurements and, hence, provides new insights about biological processes. Ratiometric methods are normally used to reduce the effects of excitation beam...
Biochem Soc Trans (2003) 31 (5): 997–1000.
Published: 01 October 2003
...V. Levi; Q. Ruan; K. Kis-Petikova; E. Gratton We describe a novel method to track fluorescent particles in three dimensions with nanometre precision and millisecond time resolution. In this method, we use our two-photon excitation microscope. The galvomotor-driven x – y scanning mirrors allow...
Biochem Soc Trans (2003) 31 (5): 1047–1051.
Published: 01 October 2003
...A.J. Bain; R.J. Marsh; D.A. Armoogum; O. Mongin; L. Porrès; M. Blanchard-Desce A new approach to time-resolved fluorescence spectroscopy based on stimulated emission depletion (STED) of two-photon excited states is presented. Combined with time-resolved detection, STED can circumvent orientational...
Biochem Soc Trans (2003) 31 (4): 851–856.
Published: 01 August 2003
...U.K. Wiegand; R.R. Duncan; J. Greaves; R.H. Chow; M.J. Shipston; D.K. Apps Large dense-core vesicles (LDCVs) were labelled in cultured bovine adrenal chromaffin cells expressing fluorescent chimaeric ‘cargo’ proteins that were targeted to these secretory vesicles. When the cells were stimulated...