... of apoptosis restoration strategies. Recent years have been prolific in the development of biosensors to visualize cyt c mitochondrial efflux in living cells, by fluorescence microscopy. These biosensors specifically detect endogenous, untagged cyt c , while showing efficient cellular uptake and reduced cell...

...Lucien E. Weiss; Tal Naor; Yoav Shechtman The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations...

... colin.campbell@ed.ac.uk ). 30 5 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 fluorescence nanosensor surface-enhanced Raman spectroscopy (SERS) ratiometric nanosensor Intracellular biological nanosensors are an important tool in the biochemist's arsenal...

...@imperial.ac.uk ). 29 4 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 co-localization single-molecule spectroscopy (CoSMoS) dynamics fluorescence resonance energy transfer (FRET) pre-mRNA splicing single-molecule fluorescence splicessomal RNA Splicing...

...Dejian Zhou Over the last 10 years, fluorescent semiconductor QD (quantum dot)–biomolecule conjugates have emerged as a powerful new sensing platform showing great potential in a wide range of applications in biosensing, environmental monitoring and disease diagnosis. The present mini-review...

... area. 1 To whom correspondence should be addressed (email paul.winyard@pms.ac.uk ). 19 7 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 biomolecular damage cell signalling fluorescence free radical mass spectrometry measurement The present...

...Balaraman Kalyanaraman HE (hydroethidine), a widely used fluorescent dye for detecting intracellular superoxide, undergoes specific oxidation and hydroxylation reactions. The reaction between HE and O 2 •− (superoxide radical) yields a diagnostic marker product, 2-hydroxyethidium. This is contrary...

... oligosaccharides)] and GSLs in biomarker discovery, where the global changes in glycosylation are probed rather than selecting individual glycoconjugates. The technique we have employed is an HPLC-based method with glycans, free or enzymatically released, fluorescently labelled with 2-AA (2-anthranilic acid) [ 20...

... should be related to the decrease in the catalytic efficiency. The instability of the fold for the mutant proteins resulted in the accumulation of an intermediate state, partly unfolded, in-between native and unfolded states. Quenching of tryptophan fluorescence by acrylamide has further revealed...

... (yellow fluorescent protein). YFP PCA was successfully applied to visualize the protein interactions of the cargo transport receptor ERGIC-53 (endoplasmic reticulum–Golgi intermediate compartment protein of 53 kDa) with its luminal interaction partner MCFD2 (multiple coagulation factor deficiency protein...

...A.M. Powl; J. Carney; P. Marius; J.M. East; A.G. Lee Interactions between a membrane protein and the lipid molecules that surround it in the membrane are important in determining the structure and function of the protein. These interactions can be pictured at the molecular level using fluorescence...

...H. Li; L. Ying; X. Ren; S. Balasubramanian; D. Klenerman Single-molecule fluorescence has the capability to detect properties buried in ensemble measurements and, hence, provides new insights about biological processes. Ratiometric methods are normally used to reduce the effects of excitation beam...

...A.J. Bain; R.J. Marsh; D.A. Armoogum; O. Mongin; L. Porrès; M. Blanchard-Desce A new approach to time-resolved fluorescence spectroscopy based on stimulated emission depletion (STED) of two-photon excited states is presented. Combined with time-resolved detection, STED can circumvent orientational...

...V. Levi; Q. Ruan; K. Kis-Petikova; E. Gratton We describe a novel method to track fluorescent particles in three dimensions with nanometre precision and millisecond time resolution. In this method, we use our two-photon excitation microscope. The galvomotor-driven x – y scanning mirrors allow...

...D.S. Lidke; P. Nagy; B.G. Barisas; R. Heintzmann; J.N. Post; K.A. Lidke; A.H.A. Clayton; D.J. Arndt-Jovin; T.M. Jovin We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy...

...U.K. Wiegand; R.R. Duncan; J. Greaves; R.H. Chow; M.J. Shipston; D.K. Apps Large dense-core vesicles (LDCVs) were labelled in cultured bovine adrenal chromaffin cells expressing fluorescent chimaeric ‘cargo’ proteins that were targeted to these secretory vesicles. When the cells were stimulated...