... and engineering. Correspondence: Matthew Wilding ( matthew.wilding@anu.edu.au ) or Colin Jackson ( colin.jackson@anu.edu.au ) 18 1 2019 18 1 2019 18 2 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 directed evolution...

... complexity from simple systems made from biological molecules–gaining a deeper understanding of life in the process. Directed evolution is a powerful tool in Synthetic Biology, able to bypass gaps in knowledge and capable of engineering even the most highly conserved biological processes. It encompasses...

... understanding of the structure–activity relationship of GTs, directed evolution continues to be a useful approach for generating additional mutants with functionality that can be screened for in a high-throughput format. Mutating the amino acid residues constituting or close to the substrate-binding sites...

... analysis is an approach to identifying quasi-species and to investigate evolutionary trajectories in the directed evolution of enzymes for novel functions. 1 To whom correspondence should be addressed (email Bengt.Mannervik@biorg.uu.se). 2 Present address: Center for Molecular Medicine...

... The Biochemical Society 2006 bacterial cytochrome P450 CYP106A2 directed evolution steroid whole-cell biocatalysis Since effective engineering of biocatalysts, in general, requires application of several techniques, it was tempting to develop methods for directed evolution of the CYP106A2 system...

... and highlight the expression of Boolean logic in gene regulation using multiple orthogonal pairs. 1 To whom correspondence should be addressed (email chin@mrc-lmb.cam.ac.uk ). 16 1 2006 © 2006 The Biochemical Society 2006 directed evolution gene regulatory circuit genetic code...