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Keywords: cyanine dye
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Biochem Soc Trans (2006) 34 (5): 979–982.
Published: 25 October 2006
... the equilibrium, YFP − +H + ↔YFPH and is activated by the intense excitation light used in fluorescence microscopy. For further details, see [ 11 ]. The cyanine dye, Cy5, also shows extensive blinking. Cy5 blinking is markedly affected by the presence of oxygen and oxygen scavengers, indicating...