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Keywords: cryo-electron tomography
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Articles
Biochem Soc Trans (2023) BST20220221.
Published: 25 January 2023
... resolution, but this is currently limited to optimal test specimens [ 15 , 16 ]. For simplicity, we will refer to SPA-based methods simply as cryo-EM. To obtain three-dimensional (3D) information on a sample without the need to average many identical copies, cryo-electron tomography (cryo-ET) can...
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Biochem Soc Trans (2022) 50 (6): 1595–1605.
Published: 24 November 2022
... underlying chemotaxis behavior is mediated by large, highly ordered protein complexes known as chemosensory arrays. For nearly two decades, cryo-electron tomography (cryoET) has been used to image chemosensory arrays, providing an increasingly detailed understanding of their structure and function...
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Biochem Soc Trans (2022) 50 (1): 95–105.
Published: 25 January 2022
... architecture of many enveloped viruses pose a challenge for the more widely used structural biology techniques, such as X-ray crystallography. Cryo-electron tomography (cryo-ET), however, is a particularly well-suited tool for overcoming the limitations associated with visualizing the irregular shapes...
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Biochem Soc Trans (2021) 49 (5): 2081–2089.
Published: 08 September 2021
... chemoreceptor array cryo-electron tomography cryoEM in-situ structure subtomogram averaging All motile bacteria and archaea examined to date possess a highly conserved chemosensory pathway that monitors the chemical environment and directs cell migration towards nutrient sources, a behavior known...
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Biochem Soc Trans (2018) 46 (4): 807–816.
Published: 22 June 2018
...Joshua Hutchings; Giulia Zanetti Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native...
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Biochem Soc Trans (2013) 41 (5): 1227–1234.
Published: 23 September 2013
...Karen M. Davies; Bertram Daum To truly understand bioenergetic processes such as ATP synthesis, membrane-bound substrate transport or flagellar rotation, systems need to be analysed in a cellular context. Cryo-ET (cryo-electron tomography) is an essential part of this process, as it is currently...