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Keywords: cryo-electron tomography
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Biochem Soc Trans (2023) BST20220221.
Published: 25 January 2023
... resolution, but this is currently limited to optimal test specimens [ 15 , 16 ]. For simplicity, we will refer to SPA-based methods simply as cryo-EM. To obtain three-dimensional (3D) information on a sample without the need to average many identical copies, cryo-electron tomography (cryo-ET) can...
Biochem Soc Trans (2022) 50 (6): 1595–1605.
Published: 24 November 2022
... underlying chemotaxis behavior is mediated by large, highly ordered protein complexes known as chemosensory arrays. For nearly two decades, cryo-electron tomography (cryoET) has been used to image chemosensory arrays, providing an increasingly detailed understanding of their structure and function...
Biochem Soc Trans (2022) 50 (1): 95–105.
Published: 25 January 2022
... architecture of many enveloped viruses pose a challenge for the more widely used structural biology techniques, such as X-ray crystallography. Cryo-electron tomography (cryo-ET), however, is a particularly well-suited tool for overcoming the limitations associated with visualizing the irregular shapes...
Biochem Soc Trans (2021) 49 (5): 2081–2089.
Published: 08 September 2021
... chemoreceptor array cryo-electron tomography cryoEM in-situ structure subtomogram averaging All motile bacteria and archaea examined to date possess a highly conserved chemosensory pathway that monitors the chemical environment and directs cell migration towards nutrient sources, a behavior known...
In Collection
Biochem Soc Trans (2018) 46 (4): 807–816.
Published: 22 June 2018
...Joshua Hutchings; Giulia Zanetti Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native...
Biochem Soc Trans (2013) 41 (5): 1227–1234.
Published: 23 September 2013
...Karen M. Davies; Bertram Daum To truly understand bioenergetic processes such as ATP synthesis, membrane-bound substrate transport or flagellar rotation, systems need to be analysed in a cellular context. Cryo-ET (cryo-electron tomography) is an essential part of this process, as it is currently...