... a transformative agreement with Individual. cannabinoids cryo-electron microscopy crystallography G-protein-coupled receptors Human endocannabinoid signaling is primarily mediated by the class A G protein-coupled receptors (GPCRs) CB1 and CB2. Class A GPCRs consist of an extracellular N-terminal...

... of Medicine in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society. cryo-electron microscopy crystallography kinesin microtubule molecular motors tubulin Kinesins are a superfamily of motor proteins that drive a variety of essential cell...

... for this article was enabled by the participation of the University of Geneva in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with Individual. cancer cell cycle cryo-electron microscopy enzyme–substrate interactions...

... article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . caveolae caveolins cell membranes cryo-electron microscopy membrane proteins protein structure Nanoscale flask-shaped...

... insights into how geometry and ABPs regulate the actin cytoskeleton limits our understanding of the molecular mechanisms that define actin cytoskeleton remodeling and, in turn, impact emerging cell migration characteristics. With the advent of cryo-electron microscopy (cryo-EM) and advanced computational...

... extensively [ 9 , 10 ]. Correspondence: John L. Rubinstein ( john.rubinstein@utoronto.ca ) 30 11 2022 3 1 2023 10 1 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 cryo-electron microscopy electron transport chain...

... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . cryo-electron microscopy molecular chaperones molecular mechanisms protein conformation All organisms depend on properly folded and functioning proteins...

...John W. Vant; Daipayan Sarkar; Jonathan Nguyen; Alexander T. Baker; Josh V. Vermaas; Abhishek Singharoy Single particle analysis cryo-electron microscopy (EM) and molecular dynamics (MD) have been complimentary methods since cryo-EM was first applied to the field of structural biology...

... have been described: contractile tails of Myoviridae -like phages; short non-contractile tails of Podoviridae -like phages; and long and flexible non-contractile tails of Siphoviridae -like phages. While early cryo-electron microscopy (cryo-EM) work elucidated the organisation of the syringe-like...

... invaluable, the ‘resolution revolution’ of cryo-electron microscopy has transformed our structural investigations, enabling large, dynamic and often transient transcription complexes to be resolved that in many cases had resisted crystallisation. In this review, we highlight the impact cryo-electron...

... of the Biochemical Society 2021 chromatin cryo-electron microscopy epigenetics eukaryotic gene expression transcription Eukaryotic cells pack their DNA (∼6 billion bases) into the small volume of the nucleus (1 × 10 2 –1 × 10 5  µm 3 ) by organizing their DNA into complex but defined structures...

.... Correspondence: Robert M. Glaeser ( rmglaeser@lbl.gov ) 6 9 2021 29 9 2021 5 10 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 cryo-electron microscopy divide and conquer structure determination In addition...

... Laboratory of Molecular Biology in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC. cryo-electron microscopy crystallography G-protein-coupled receptors G protein-coupled receptors (GPCRs) are the largest...

... esterified variant of SMA [ 27 ]. DIBMA is a 1 : 1 alternating polymer of diisobutylene and maleic acid. Approaches for the determination of high-resolution protein structure include X-ray crystallography, NMR and cryo-electron microscopy (cryo-EM). Broecker et al. reported the first X-ray structure...

... biology, but recent advances in cryo-electron microscopy and cryo-electron tomography have further uncovered exciting new snapshots of reconstituted retromer structures. These new structures reveal retromer assembles into an arch-shaped scaffold and suggest the scaffold may be flexible and adaptable...

... Society under a transformative agreement with JISC. biochemistry cryo-electron microscopy DNA replication in silico reconstitution single molecule Accurate transfer of genetic information from parental to daughter cells requires that chromosome replication is finely tuned, so that DNA...

.... Biol. 25 , 616 – 622 10.1038/s41594-018-0085-x AAA proteins cryo-electron microscopy endosomal sorting protein disassembly protein structure E ndosomal S orting C omplex R equired for T ransport (ESCRT) pathways drive membrane fission in numerous cellular...

...-resolution structural information that was mostly limited to individual proteins and smaller sub-complexes. Recent developments in the field of cryo-electron microscopy allowed the visualisation of fully assembled yeast and human spliceosomes, providing unprecedented insights into substrate recognition...

... that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility. cryo-electron microscopy cryo-electron tomography subtomogram averaging Figure 1. An overview of structural...

... 2017 © 2017 The Author(s) 2017 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . cowpea mosaic virus cryo-electron microscopy mutagenesis transient...

... Press Limited on behalf of the Biochemical Society 2016 cryo-electron microscopy eukaryotic RNA polymerase complex integrated structural biology multiprotein assembly X-ray crystallography Our laboratory studies the mechanisms of gene transcription and its regulation in eukaryotic...

...Robert J.C. Gilbert; Ian Brierley; John E.G. McCarthy High-resolution structures have given an extremely detailed view of aspects of ribosomes, including some near-functional states. Here, we review the importance of cryo-electron microscopy, among other techniques, in giving an understanding...

... The Biochemical Society 2006 ADP-ribosylation cryo-electron microscopy diphthamide diphtheria toxin elongation factor 2 (EF2) exotoxin A In the elongation cycle of protein synthesis, elongation factor 1A delivers aminoacylated tRNA to the ribosomal A-site, whereafter the peptidyl transferase...