... regulatory mechanisms that result in compartmentalized signalling. The use of fluorescent probes to monitor cAMP in intact, living cells have been instrumental in furthering our appreciation of this ancestral and ubiquitous pathway and unexpected details of the nano-architecture of the cAMP signalling...

.... It is now apparent that GPCR signalling is restricted to highly organized compartments within the cell, and that GPCRs activate distinct signalling pathways once internalized. A high-resolution understanding of how compartmentalized signalling is controlled will probably provide unique opportunities...

... To whom correspondence should be addressed (email khalid.naseem@hyms.ac.uk ). 19 12 2013 © The Authors Journal compilation © 2014 Biochemical Society 2014 A-kinase-anchoring protein (AKAP) cAMP compartmentalization phosphodiesterase (PDE) platelet protein kinase A (PKA...

...Konstantinos Lefkimmiatis Mitochondria are highly dynamic organelles comprising at least three distinct areas, the OMM (outer mitochondrial membrane), the IMS (intermembrane space) and the mitochondrial matrix. Physical compartmentalization allows these organelles to host different functional...

... Ras genes are not functionally redundant; this has been attributed to their distinctive trafficking and localization profiles. A palmitoylation cycle controls the correct compartmentalization of H-Ras and N-Ras. We review recent data that reveal how this cycle can be regulated by membrane organization...

... regulation of cAMP signalling by SOCE. 1 email dw212@cam.ac.uk 14 6 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 adenylate cyclase (AC) A-kinase-anchoring protein (AKAP) biosensor compartmentalization Orai1 store-operated calcium entry (SOCE...

... compartmentalization extracellular-signal-regulated kinase (ERK) phosphodiesterase (PDE) protein kinase A (PKA) Figure 2 AKAP450 targets PKA Type II and PDE4D3 to the centrosomal region in Sertoli cells A similar mechanism operates in cardiomyocytes, where mAKAP binds and targets both PDE4D3 and PKA Type...

... 2006 The Biochemical Society 2006 A-kinase-anchoring protein (AKAP) cAMP cycling compartmentalization protein kinase A (PKA) Although the investigation of cAMP signalling has been underway for over 50 years, it was not until the early 1980s that the compartmentalized modulation...

.... Mol. Cell. Biol. 1999 19 6286 6296 11 Dodge K.L. Khouangsathiene S. Kapiloff M.S. Mouton R. Hill E.V. Houslay M.D. Langeberg L.K. Scott J.D. EMBO J. 2001 20 1921 1930 cAMP compartmentalization heart failure phosphodiesterase 3B (PDE3B...

... in the apoplasm were measured, whereas non-aqueous fractionation was used for compartmental analysis within cells [ 8 , 9 ]. Still, none of the above approaches permits real-time measurements in living cells. On the other hand, most techniques enabling dynamic measurements only offer limited spatial resolution...