... of the individual functional domains. Finally, we discuss the use of these signaling proteins as drug targets or as sensing devices in whole-cell biosensors with medical and biotechnological applications. A TCS is typically comprised by a sensor histidine kinase (HK) and a cognate response regulator (RR). HKs...

... © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 ACEMBL AMPK ATP ADP AMP biosensor cellular energetics fluorescence resonance energy transfer Energy metabolism is key to cell survival and homeostasis. Metabolic and energy...

.... Correspondence: Viacheslav O. Nikolaev ( v.nikolaev@uke.de ) 22 7 2019 20 9 2019 24 9 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 biosensor cardiac cGMP fluorescence resonance energy transfer microdomain 3′,5...

...@vt.edu ) 22 4 2015 © 2015 Authors; published by Portland Press Limited 2015 alternative protein scaffold biosensor imaging agent molecular recognition protein design repeat proteins The sensitivity and specificity of biosensors and imaging agents are decidedly dependent...

... of synthetic biology, it is now possible to design and implement biosensors that consist of biological macromolecules and are able to report on the intracellular environment of cells. The use of fluorescent reporter signals allows non-invasive, non-destructive and online monitoring of the culture, which...

... is a brief account of the recent developments in QD–NA (nucleic acid), particularly NA aptamer, conjugate-based biosensors using the FRET (Förster resonance energy transfer) readout mechanism. It starts with a brief introduction to the NA aptamer and QD-FRET, followed by example approaches to compact QD–DNA...

...-resolution targeted biosensors for Ca 2+ and cAMP, have provided the first detailed insight into the organization of the cellular microdomains associated with Ca 2+ -regulated ACs. In the present review, I summarize the findings that have helped to provide our most definitive understanding of the selective...

... an overview of our early-stage experimental data and methodology, examine the potential of fibre gratings for use as biosensors to monitor both nucleic acid and other biomolecular interactions and then give a summary of the theory and fabrication of fibre gratings from a biological standpoint. Finally...

.... Real-time imaging of fluorescent biosensors is making it possible to visualize cAMP dynamics directly as they happen in intact, living cells, providing important and original insights for our understanding of the spatiotemporal organization of the cAMP/PKA (protein kinase A) signalling pathway. One...