...Changyi Zhang; Bin Tian; Suming Li; Xiang Ao; Kevin Dalgaard; Serkan Gökce; Yunxiang Liang; Qunxin She Recently, a novel gene-deletion method was developed for the crenarchaeal model Sulfolobus islandicus , which is a suitable tool for addressing gene essentiality in depth. Using this technique, we...

...Changyi Zhang; David J. Krause; Rachel J. Whitaker Sulfolobus islandicus has been developed as a model system for combining approaches of evolutionary and molecular biology in Archaea. We describe how the application of this interdisciplinary approach can lead to novel hypotheses derived from...

... archaeon gene knockout hyperthermophile marker gene plasmid Sulfolobus islandicus Sulfolobus is a genus of hyperthermophilic acidophiles that thrives in terrestrial acidic hot springs worldwide. These organisms are aerobic heterotrophs, growing optimally in a relatively simple medium at 75...