... on their major chromosome, in stark contrast with bacteria, which have a single replication origin. Two of these replication origins of Haloferax volcanii have been studied in detail and turned out to have very different properties. The chromosome copy number appears to be regulated in response to growth phases...

... Society 2019 archaea Haloferax volcanii transcription termination Pro- and eukaryotes use multisubunit RNA polymerases (RNAPs) sharing a common ancestry [ 1 ]. Homology on the level of amino acid sequence is quite low, but a comparison of the overall structure and topology as well...

... in the halophilic archaeon Haloferax volcanii . H. volcanii encodes a subtype I-B CRISPR–Cas system and the defence can be triggered by a plasmid-based invader. Six different target-interference motifs are recognized by the Haloferax defence and a 9-nt non-contiguous seed sequence is essential. The repeat sequence...

... details of many of the steps involved in adaptation and defence are yet to be understood. In the present paper, we summarize our current knowledge about the CRISPR/Cas system in Haloferax volcanii , an extremely halophilic archaeon that was isolated from the Dead Sea. H. volcanii encodes a type I-B CRISPR...

...Lina Kaminski; Shai Naparstek; Lina Kandiba; Chen Cohen-Rosenzweig; Adi Arbiv; Zvia Konrad; Jerry Eichler Although performed by members of all three domains of life, the archaeal version of N-glycosylation remains the least understood. Studies on Haloferax volcanii have, however, begun to correct...

... recombination in a saltern population of Halorubrum Science 2004 306 1928 1929 8 Soppa J. Optimization of the 5-bromo-2'-deoxyuridine selection and its application for the isolation of nitrate respiration-deficient mutants of Haloferax volcanii J. Microbiol. Methods 1998 34 41 48 9...

... knowledge about sRNAs and their function in the halophilic archaeon Haloferax volcanii . Using two different experimental approaches, 111 intergenic and 38 antisense sRNAs were identified, as well as 42 tRFs (tRNA-derived fragments). Observation of differential expression under various conditions suggests...

... for the presence of sRNAs in the model archaeon Haloferax volcanii , resulting in more than 150 putative sRNA genes being identified. Northern blot analyses were used to study (differential) expression of sRNA genes. Several chromosomal deletion mutants of sRNA genes were generated and compared with the wild-type...

... genetic and molecular systems have been established. We are developing such systems using Haloferax volcanii as a model organism, as it can be cultivated in the laboratory with ease and offers great potential for establishing tractable and informative genetic systems. 1 To whom correspondence should...