... recently, its function remained unclear. AP-4 associates with the trans -Golgi network (TGN) through interaction with small GTPases of the ARF family and recognizes transmembrane proteins (i.e. cargos) having specific sorting signals in their cytosolic domains. Recent studies identified accessory proteins...

... © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 autophagy Golgi human genetics kinase Rab GTPases vesicles Early studies of LRRK2 noted that it was distributed in cytosolic and vesicular compartments in cells, but was largely...

... the cytoskeleton dynamics with vesicle trafficking during cytokinesis are poorly understood. The highly conserved Golgi phosphoprotein 3 (GOLPH3), functions as a phosphatidylinositol 4-phosphate (PI4P) effector at the Golgi. Recent studies have suggested that GOLPH3 is up-regulated in several cancers...

...Michelle L. Villasmil; Vytas A. Bankaitis; Carl J. Mousley The Kes1 OSBP (oxysterol-binding protein) is a key regulator of membrane trafficking through the TGN ( trans- Golgi network) and endosomal membranes. We demonstrated recently that Kes1 acts as a sterol-regulated rheostat for TGN/endosomal...

...Hailan Piao; Peter Mayinger PtdIns4 P is a key regulator of the secretory pathway and plays an essential role in trafficking from the Golgi. Our recent work demonstrated that spatial control of PtdIns4 P at the ER (endoplasmic reticulum) and Golgi co-ordinates secretion with cell growth...

...Marina Mikhaylova; Pasham Parameshwar Reddy; Michael R. Kreutz The regulated local synthesis of PtdIns4 P and PtdIns(4,5) P 2 is crucial for TGN ( trans -Golgi network)–plasma membrane trafficking. The activity of PI4Kβ (phosphoinositide 4-kinase IIIβ) at the Golgi membrane is a first mandatory...

... are the most divergent and most studied human Arf proteins that localize predominantly to the Golgi complex and plasma membrane respectively. We have been studying the targeting of Arf1 and Arf6 to these specific compartments and the common and divergent activities that they exert on these membranes. We have...

... that has been instrumental in deciphering the molecular principles of membrane traffic at the Golgi. BFA targets a low-affinity Arf–Sec7 intermediate of the exchange reaction. It binds at the Arf-GDP/Sec7 interface, thus freezing the complex in an abortive conformation that cannot proceed to nucleotide...

... family members, regulation of vesicle traffic and microtubule dynamics, diverged from one function of Arfs in the earliest cells that has continued to branch and allow additional levels of regulation. ADP-ribosylation factor (Arf) Arf-like (Arl) cytokinesis Golgi membrane traffic microtubule...

... ). 3 3 2005 © 2005 The Biochemical Society 2005 Cdc42 Golgi guanine nucleotide-exchange factor (GEF) HeLa cell lamellipodiae Rho GDP dissociation inhibitor (rhoGDI) Small G-proteins of the Rho family regulate many features of the normal cell such as polarity, motility...

... nascent Ras proteins transit endomembranes en route to the PM (plasma membrane) and that at steady-state N-Ras and H-Ras are highly expressed on the Golgi apparatus. To test the hypothesis that Ras proteins on internal membranes can signal, we developed a fluorescent probe that reports when and where...

..., and which is localized to the Golgi in all species so far examined. The best-characterized effectors of Arl1 are coiled-coil proteins which share a C-terminal GRIP domain, but other apparent effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps fifty-three) vesicle-tethering complex...

... activities at the Golgi and plasma membrane respectively. Chimaeras were made between Arf1 and Arf6 in order to identify regions of the protein that contributed to targeting and function. Whereas Arf6 is targeted to the plasma membrane through multiple regions along the protein, we have found a Golgi...

... ARL stands for ARF-like) encoded in the T. brucei and T. cruzi genomes and ten in L. major . The T. brucei ARL1 protein is expressed only in the mammalian bloodstream form of the parasite, in which it is localized to the Golgi apparatus. RNAi (RNA interference) has been used to demonstrate that ARL1...

... (email rnc8@cornell.edu ). 9 3 2005 © 2005 The Biochemical Society 2005 Golgi prenylation Rab GTPase Rab-GDI YIP1 family Yip1p If YIP1 family members display in vitro GDF activity, does this make them an indispensable part of the Rab localization pathway in vivo ? What...

...: a Biochemical Society Focused Meeting held at Rothamsted Research, Harpenden, 25 November 2003 25 November 2003 © 2004 Biochemical Society 2004 Golgi isotope-coded affinity tag (ICAT) membrane protein organelle quantitative proteomics Abbreviations used: 2D, two-dimensional...