...Pascal D. Vos; Aleksandra Filipovska; Oliver Rackham The discovery of CRISPR–Cas9 and its widespread use has revolutionised and propelled research in biological sciences. Although the ability to target Cas9's nuclease activity to specific sites via an easily designed guide RNA (gRNA) has made...

...Jurre A. Steens; Carl Raymund P. Salazar; Raymond H.J. Staals Type III CRISPR–Cas systems make use of a multi-subunit effector complex to target foreign (m)RNA transcripts complementary to the guide/CRISPR RNA (crRNA). Base-pairing of the target RNA with specialized regions in the crRNA not only...

... homology, which is compounded by low genetic tractability compared with many model organisms. In recent years technical breakthroughs have made forward and reverse genome-scale screens in Plasmodium possible. Furthermore, the adaptation of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR...

...Martin Pal; Marco J. Herold CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage...

... for synthetic circuit construction but some of their inherent constraints, such as insufficient modularity, orthogonality and programmability, limit progress in such forward-engineering endeavors. Here we review how CRISPR (clustered regularly interspaced short palindromic repeats) technology offers new...

...Claudio Hidalgo-Cantabrana; Rodolphe Barrangou CRISPR-Cas constitutes the adaptive immune system of bacteria and archaea. This RNA-mediated sequence-specific recognition and targeting machinery has been used broadly for diverse applications in a wide range of organisms across the tree of life...

...Thomas Swartjes; Raymond H.J. Staals; John van der Oost Discovered as an adaptive immune system of prokaryotes, CRISPR–Cas provides many promising applications. DNA-cleaving Cas enzymes like Cas9 and Cas12a, are of great interest for genome editing. The specificity of these DNA nucleases...

... of action and secondly discussing how these RNA components have been exploited to rewire bacterial regulatory circuitry. 26 7 2019 13 9 2019 16 9 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 5′UTR regulator CRISPR gene...

... CRISPR DNA fluorescence microscopy single molecule super resolution Observing DNA inside cells has an increasing number of applications ranging from diagnostics [ 1 ], cancer and cell viability research [ 2 – 7 ] to more fundamental studies on chromatin structure and dynamics [ 8 – 11...