... of the Biochemical Society 2021 ABC transporter allosteric communication ATP binding and hydrolysis conformational transition coupling helix substrate ATP-binding cassette (ABC) transporters are membrane proteins that utilize the energies of ATP binding and hydrolysis to transport various...

... and closing of its anion-selective pore are linked to ATP binding and hydrolysis at CFTR's two NBDs (nucleotide-binding domains), NBD1 and NBD2. Isolated NBDs of prokaryotic ABC proteins form homodimers upon binding ATP, but separate after hydrolysis of the ATP. By combining mutagenesis with single-channel...