... on behalf of the Biochemical Society 2020 ACEMBL AMPK ATP ADP AMP biosensor cellular energetics fluorescence resonance energy transfer Our AMPfret sensor is based on the native heterotrimeric AMPK itself, thus directly reporting on AMPK activation mechanisms as naturally evolved...

...Kirk A. Taylor; Joy R. Wright; Martyn P. Mahaut-Smith Pannexin-1 (Panx1) forms anion-selective channels with a permeability up to 1 kDa and represents a pathway for the release of cytosolic ATP. Several structurally similar connexin (Cx) proteins have been identified in platelets and shown to play...

..., the signalling pathways eliciting glycogen degradation inside astrocytes are themselves energy-demanding processes, a fact that has been emphasized in recent studies, demonstrating dependence of these signalling mechanisms on glycogenolytic ATP. Although the somata of noradrenergic neurons are confined to two...

..., which maintain the well-being and function of neurons. Still, the study of age-related changes in neuron–glia signalling is far from complete. We have shown previously that cortical astrocytes are capable of releasing ATP by a quantal soluble N -ethylmaleimide-sensitive factor-attachment protein...

... by the adenine ring of ATP. In the present paper, we show how the spine concept can be applied to B-Raf, specifically to engineer a kinase-dead pseudokinase. To achieve this, we mutated one of the C-spine residues in the N-lobe (N-terminal lobe), Ala 481 , to phenylalanine. This mutant cannot bind ATP...

... and ATP insufficiency play closely interlocked roles that may be investigated with primary neuronal cultures. 1 email dnicholls@buckinstitute.org 4 7 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 ATP excitotoxicity glutamate ischaemia mitochondrion...

...G.L. Ackland; V. Kasymov; A.V. Gourine The purine nucleotide ATP mediates several distinct forms of sensory transduction in both the peripheral and central nervous systems. These processes share common mechanisms that involve the release of ATP to activate ionotropic P2X and/or metabotropic P2Y...

... and the current understanding of the signalling pathways from receptor to RONS generators. BzATP 2′-3′- O -(4-benzoyl)benzoyl ATP JNK1 c-Jun N-terminal kinase 1 MAPK mitogen-activated protein kinase oATP oxidized ATP P2XR P2X receptor P2X 7 R P2X 7 receptor P2YR P2Y...

...J.A. Eldred; J. Sanderson; M. Wormstone; J.R. Reddan; G. Duncan ATP release has been shown to occur following stimulation in several cellular systems. This study was undertaken to determine if lens and retinal epithelial cells release ATP in response to physiological stresses and to elucidate...

...Jenny A. Wilkinson; Ron Jacob [Ca 2+ ] i (cytosolic [Ca 2+ ]) and OS (oxidative stress) were measured simultaneously in calf pulmonary artery endothelial cells using fura-2 and carboxy-2´,7´-dichlorodihydrofluorescein. ATP stimulated a [Ca 2+ ] i increase that was followed a few seconds later...

...M. J. Kushmerick; K. E. Conley Understanding muscle energetics is a problem in optimizing supply of ATP to the demands of ATPases. The complexity of reactions and their fluxes to achieve this balance is greatly reduced by recognizing constraints imposed by the integration of common metabolites...