... elements (CRE) and transcription factor binding sites and, when combined with transcriptomics, can reveal gene regulatory networks (GRNs) of expressed genes. Chromatin accessibility mapping is a powerful approach and can be performed using ATAC-sequencing (ATAC-seq), whereby Tn5 transposase inserts...

... remain in the aqueous phase during phenol:chloroform extraction and are sequenced. ( C ) MNase-seq: MNase is used to digest away linker DNA regions and nucleosomal DNA is sequenced. ( D ) ATAC-seq: A hyperactive Tn5 transposase simultaneously cleaves and inserts DNA adapters at accessible regions...