Cytochrome c1 is a subunit of ubiquinol: cytochrome c reductase (E.C.1.10.2.2.). In Neurospora grown in the presence of chloramphenicol, the subunit is inserted only into the bilayer of the mitochondrial inner membranes without associating with other proteins. By limited proteolytic digestion of the cytochrome c1-Triton complex with chymotrypsin, a large water soluble monomeric cytochrome c1 fragment was prepared. The 24,000 Mr fragment and the 31,000 Mr cytochrome c1 have the same spectra and cytochrome c binding properties. These results are used to propose a two-part model for cytochrome c1. A large hydrophilic part extends from the mitochondrial inner membrane into the intermembrane space. This part carries the heme and interacts with cytochrome c. A second, smaller hydrophobic part anchors the cytochrome c1 to the bilayer.
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April 1981
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Conference Abstract|
April 01 1981
MEMBRANE BOUND AND WATER SOLUBLE CYTOCHROME C1 FROM NEUROSPORA MITOCHONDRIA
Yun Li;
Yun Li
1European Molecular Biology Laboratory, Postfach 10.2209, 6900 Heidelberg, FRG
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Kevin Leonard;
Kevin Leonard
1European Molecular Biology Laboratory, Postfach 10.2209, 6900 Heidelberg, FRG
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Hanns Weiss
Hanns Weiss
1European Molecular Biology Laboratory, Postfach 10.2209, 6900 Heidelberg, FRG
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 1981 Biochemical Society
1981
Biochem Soc Trans (1981) 9 (2): 358P.
Citation
Yun Li, Kevin Leonard, Hanns Weiss; MEMBRANE BOUND AND WATER SOLUBLE CYTOCHROME C1 FROM NEUROSPORA MITOCHONDRIA. Biochem Soc Trans 1 April 1981; 9 (2): 358P. doi: https://doi.org/10.1042/bst009358pe
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