Membranes of cells are active barriers, in which membrane proteins perform essential remodelling, transport and recognition functions that are vital to cells. Membrane proteins are key regulatory components of cells and represent essential targets for the modulation of cell function and pharmacological intervention. However, novel folds, low molarity and the need for lipid membrane support present serious challenges to the characterisation of their structure and interactions. We describe the use of solid state NMR as a versatile and informative approach for membrane and membrane protein studies, which uniquely provides information on structure, interactions and dynamics of membrane proteins. High resolution approaches are discussed in conjunction with applications of NMR methods to studies of membrane lipid and protein structure and interactions. Signal enhancement in high resolution NMR spectra through DNP is discussed as a tool for whole cell and interaction studies.
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August 2021
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Glycoproteomics is the tool of choice in glycobiology to decipher the role of protein glycosylation in health and disease in a system-wide context for integration into multi-omics studies. For a hitchhiker's guide to glcoproteomics, see the review by Oliveira and colleagues (pp. 1623–1642). Cover artwork provided by Daniel Kolarich.
Review Article|
August 16 2021
Solid state NMR of membrane proteins: methods and applications
Vivien Yeh;
Vivien Yeh
School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, U.K.
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Boyan B. Bonev
School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, U.K.
Correspondence: Boyan B. Bonev (boyan.bonev@nottingham.ac.uk)
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Publisher: Portland Press Ltd
Received:
June 01 2021
Revision Received:
July 17 2021
Accepted:
July 27 2021
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2021
Biochem Soc Trans (2021) 49 (4): 1505–1513.
Article history
Received:
June 01 2021
Revision Received:
July 17 2021
Accepted:
July 27 2021
Citation
Vivien Yeh, Boyan B. Bonev; Solid state NMR of membrane proteins: methods and applications. Biochem Soc Trans 27 August 2021; 49 (4): 1505–1513. doi: https://doi.org/10.1042/BST20200070
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